We narrowed to 1,540 results for: SEU

1. pCPP5233

   Plasmid

   #128715

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

   Insert

   shcM-hopM1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPP5060

   Plasmid

   #128716

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

   Insert

   shcN-hopN1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCPP3373

   Plasmid

   #128718

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopQ1-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Synonymous point mutation in the wobble position…

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSG068

   Plasmid

   #214259

   Purpose

   pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI

   Depositor

   Ute Romling

   Article

   Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

   Insert

   Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   18 nucleotide 5' of the start codon (Shine-D…

   Promoter

   araBAD promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSG059

   Plasmid

   #214262

   Purpose

   PaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+)

   Depositor

   Ute Romling

   Article

   Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

   Insert

   tLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
   

   Use

   Tags

   6x His-tag and 6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pACB143

   Plasmid

   #222214

   Purpose

   For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   vanB(A24P)
   

   Use

   Tags

   N-terminal, thrombin-cleavable 6x His tag

   Expression

   Bacterial

   Mutation

   Changed Alanine 24 to Proline

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSN95

   Plasmid

   #222220

   Purpose

   For recombinant expression of vanA (PP_3736 from Pseudomonas putida) with a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   vanA putida (PP_RS19450 Pseudomonas putida KT2440)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACB109

   Plasmid

   #222208

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (K10N)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Lys10 to Asn

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACB110

   Plasmid

   #222209

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (G258D)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Gly258 to Aspartate

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACB111

    Plasmid

    #222210

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(G76V)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Changed glycine 76 to valine

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pACB112

    Plasmid

    #222211

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(S11R)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Changed Serine 11 to Arginine

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pACB113

    Plasmid

    #222212

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(W15C)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Changed Tryptophan 15 to Cysteine

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUN378 - pTRV2-CrGLK

    Plasmid

    #203888

    Purpose

    Viral silencing plasmid targeting the CrGLK gene from C. roseus.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    CrGLK fragment
    

    Use

    RNAi

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET20b_Pa-PiuA

    Plasmid

    #128955

    Purpose

    expresses Pseudomonas aeruginosa PiuA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET20b_Pa-PirA

    Plasmid

    #128956

    Purpose

    expresses Pseudomonas aeruginosa PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSG071

    Plasmid

    #214257

    Purpose

    Hybrid PaFtsH1NFtsH2 protease: PaftsH2SD -PaftsH21-462-PaftsH1454-1920

    Depositor

    Ute Romling

    Article

    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

    Insert

    Hybrid PaFtsH protease PaFtsH1NFtsH2 of Pseudomonas aeruginosa SG17M
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…

    Promoter

    araBAD promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSG075

    Plasmid

    #214258

    Purpose

    Hybrid PaFtsH2NFtsH1 protease: PaftsH2SD -PaftsH11-453-PaftsH2463-1881 in pJN105

    Depositor

    Ute Romling

    Article

    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

    Insert

    Hybrid PaFtsH protease PaFtsH2NFtsH1 of Pseudomonas aeruginosa SG17M
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…

    Promoter

    araBAD promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSG086

    Plasmid

    #214263

    Purpose

    PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

    Depositor

    Ute Romling

    Article

    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

    Insert

    Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    substitution of 31 amino acids of FtsH2 (F124RRFA…

    Promoter

    T7 promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUN381 - pTRV2-CHLH

    Plasmid

    #203886

    Purpose

    Viral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    CHLH fragment
    

    Use

    RNAi

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUN1243 - pL0_pT3O (pro + 5U)

    Plasmid

    #203891

    Purpose

    T3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T3O promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge