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p11d-tRPA-32Asp8

Plasmid

#102617

Purpose

Expression of human RPA with aspartic acid substituted phosphorylation domain

Depositor

Marc Wold

Publication

Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

Inserts

RPA1 (RPA1 Human)
RPA3 (RPA3 )
RPA2 (RPA2 )

Expression

Bacterial

Mutation

with aspartic acid substituted phosphorylation do…

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-hsRPA70 (MSW#473)

Plasmid

#208067

Purpose

Expression of GFP-tagged hsRPA1 in human cells

Depositor

Marc Wold

Publication

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA1 (RPA1 Human)

Tags

GFP

Expression

Mammalian

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRPA70-IRES-RPA32-IRES-RPA14

Plasmid

#211432

Purpose

mammalian expression of human RPA70 and RPA32 and RPA14

Depositor

Helmut Pospiech

Insert

RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)

Expression

Mammalian

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-N

Plasmid

#128308

Purpose

Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 N terminal fragment (OB fold) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (1–138 aa)

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pmVen-RPA70-IRES-RPA32-IRES-RPA14

Plasmid

#211431

Purpose

mammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14

Depositor

Helmut Pospiech

Insert

RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)

Tags

mVenus (on RPA70)

Expression

Mammalian

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-C

Plasmid

#128311

Purpose

Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2NhNABP1C

Plasmid

#128313

Purpose

Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP2N+hNABP1C (NABP2, NABP1 Human)

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP2N+hNABP1C, Y85C (please se…

Promoter

T7

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDX3X-E285K

Plasmid

#116226

Purpose

Lentiviral expression of DDX3X E285K

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

E285K

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA (MSW#111)

Plasmid

#208066

Purpose

expression of human Replication Protein A in E. coli

Depositor

Marc Wold

Publication

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)

Expression

Bacterial

Mutation

RPA32 has a point mutation Thr88(ACC) -> Ile(A…

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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p11d-aRPA (MSW#712)

Plasmid

#208078

Purpose

Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

Depositor

Marc Wold

Publication

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDX3X-S412F

Plasmid

#116227

Purpose

Lentiviral expression of DDX3X S412F

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

S412F

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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DDX3X in pENTR

Plasmid

#216572

Purpose

gateway cloning donor vector containing DDX3X

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway Cloning

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-C

Plasmid

#128309

Purpose

Express hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 C-terminal domain (122–211 aa) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

C-terminal domain (122–211 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1NhNABP2C

Plasmid

#128312

Purpose

Express chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP1N+hNABP2C (NABP1, NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP1N+hNABP2C

Promoter

T7

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMS22

Plasmid

#129710

Purpose

Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)

Tags

10-Histidine tag cleavable with PreScission Prote…

Expression

Bacterial

Mutation

RssB E134G:E135S:E136G:E137S

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEF 3-GUS-2

Plasmid

#20104

Depositor

Antonio Richart

Insert

GUS (gus )

Use

Synthetic Biology; Plant gateway vector

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pREC1010-Kva1

Plasmid

#240684

Purpose

RSF1010 origin of replication plasmid containing Kva1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Kva1 RT,Kva1 ncRNA, Beta and EcSSB

Expression

Bacterial

Mutation

WT

Available since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRECBRR1-Kva1

Plasmid

#240703

Purpose

inverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoter

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Kva1 RT,Kva1 ncRNA, PaRecT and PaSSB

Expression

Bacterial

Mutation

Inverted BRR1 origin of replication

Available since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRPA70-IRES-Ven-RPA32-IRES-RPA14

Plasmid

#211430

Purpose

mammalian expression of human RPA32 N-terminally fused to mVenus and RPA70 and RPA14

Depositor

Helmut Pospiech

Insert

RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)

Tags

mVenus (on RPA32)

Expression

Mammalian

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-N

Plasmid

#128310

Purpose

Express hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (OB fold, 1–140 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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