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pUCHR-inLuc

Plasmid

#58955

Purpose

HIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCRU5HT1-inLuc

Plasmid

#58954

Purpose

HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Retroviral

Tags

Expression

Mutation

Promoter

CMV/R/U5

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221-olig1

Plasmid

#192744

Purpose

Gateway entry vector encoding zebrafish olig1

Depositor

Summer Thyme

Insert

olig1 (olig1 Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

F106L

Promoter

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-sCAG-eGFP-RAB11

Plasmid

#203799

Purpose

AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Insert

RAB11 (RAB11A Human)

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-SYN-TdTomato-RAB11

Plasmid

#203732

Purpose

AAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Insert

RAB11 (RAB11A Human)

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

Promoter

Human synapsin promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-SYN-eGFP-RAB11

Plasmid

#203731

Purpose

AAV vector plasmid expressing human RAB11 fused to eGFP under the human synapsin (SYN) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Insert

RAB11 (RAB11A Human)

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Human synapsin promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-nrip1b

Plasmid

#192732

Purpose

Gateway entry vector encoding zebrafish nrip1b

Depositor

Summer Thyme

Insert

nrip1b (nrip1b Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

L661P (rs511527097); S720P; A801T; K862E; A863P; …

Promoter

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-CDK4shRNA1

Plasmid

#220574

Purpose

To inducibly knockdown CDK4 expression

Depositor

Sean Lee

Insert

CDK4 shRNA1 (CDK4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-CDK4shRNA2

Plasmid

#220575

Purpose

To inducibly knockdown CDK4 expression

Depositor

Sean Lee

Insert

CDK4 shRNA2 (CDK4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-dyrk1aa

Plasmid

#192741

Purpose

Gateway entry vector encoding zebrafish dyrk1aa

Depositor

Summer Thyme

Insert

dyrk1aa (dyrk1aa Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj6

Plasmid

#192725

Purpose

Gateway entry vector encoding zebrafish kcnj6

Depositor

Summer Thyme

Insert

kcnj6 (kcnj6 Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

5' insertion of ATGGCCAAGCTGACAGAATCC, ident…

Promoter

None

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR223 Rab11A WT-STOP

Plasmid

#228030

Purpose

DONR vector for shuttling Rab11A WT into DEST vector. Contains a STOP codon.

Depositor

Wade Harper

Insert

Rab11A (RAB11A Human)

Use

Gateway donr

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-CALU-WPRE-UbC-mCherry

Plasmid

#225952

Purpose

Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

Depositor

Evan Reid, Bart Nieuwenhuis, Richard Eva

Inserts

CALU (CALU Human)
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV and UbC

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBigTB

Plasmid

#149432

Purpose

shuttle vector with blasticidin-S resistance into ROSA26 targetting vectors

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-CALU-WPRE-UbC-Emerald

Plasmid

#225951

Purpose

Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Evan Reid, Bart Nieuwenhuis, Richard Eva

Inserts

CALU (CALU Human)
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV and UbC

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221-appa

Plasmid

#192745

Purpose

Gateway entry vector encoding zebrafish appa

Depositor

Summer Thyme

Insert

appa (appa Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

S47N

Promoter

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR223 Rab11A S25N-STOP

Plasmid

#228032

Purpose

DONR vector for shuttling Rab11A S25N (Dominant negative mutation) into DEST vector. Contains a STOP codon.

Depositor

Wade Harper

Insert

Rab11A (RAB11A Human)

Use

Gateway donr

Tags

Expression

Mutation

S25N

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223 Rab11A Q70L-STOP

Plasmid

#228033

Purpose

DONR vector for shuttling Rab11A Q70L (constitutively active mutation) into DEST vector. Contains a STOP codon.

Depositor

Wade Harper

Insert

Rab11A (RAB11A Human)

Use

Gateway donr

Tags

Expression

Mutation

Q70L

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223 Rab11A S20V-STOP

Plasmid

#228031

Purpose

DONR vector for shuttling Rab11A S20V (constitutively active mutation) into DEST vector. Contains a STOP codon.

Depositor

Wade Harper

Insert

Rab11A (RAB11A Human)

Use

Gateway donr

Tags

Expression

Mutation

S20V

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-kcnj13

Plasmid

#173143

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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