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Plasmid#90503PurposeExpresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNRDepositorInsertmito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-244
Plasmid#90502PurposeExpresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - IRES - MTS - tFD - P2A - MTS - tFNRDepositorInsertmito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-112
Plasmid#90494PurposepcDNA3 - pCMV - MTAD - PIF3, expresses Phytochrome interacting factor 3 (PIF3) and minimal transactivation domain (MTAD), under CMV promoterDepositorInsertMTAD-PIF3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
gfp-Arkadia/ RNF111 deltaRING
Plasmid#112229PurposeExpresses GFP-tagged enzymatically inactive (delta RING) Arkadia in mammalian cellsDepositorInsert(Arkadia)Rnf111 (Rnf111 Mouse)
UseTagsGFPExpressionMammalianMutationdelta RING domain deletion (Deletion of the last …PromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i5-msfGFP
Plasmid#180327Purposemammalian expression of human SEPT9_i5 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v7 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPKm-231
Plasmid#90500PurposepSIN - EF-1alpha - MTS - tFd - P2A - MTS - tFNR, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd) and Ferredoxin-NADP(+) oxireductase (FNR), under EF-1alpha promoterDepositorInsertmito-tFD and mito-tFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-232
Plasmid#90501PurposepSIN - EF-1alpha - MTS tHO1 - P2A - MTS - tPCYA, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase, under EF-1alpha promoterDepositorInsertmito-tHO1 and mito-tPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-234
Plasmid#90507PurposepSIN - EF-1alpha - MTS sHO1 - P2A - MTS - sPCYA, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoterDepositorInsertmito-sHO1, mito-sPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
OPEN-HLA-A*11:01-BirA
Plasmid#235135PurposeE. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.DepositorInsertOPEN-HLA-A*11:01 (HLA-A Human)
UseTagsBirAExpressionBacterialMutationG120CPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEXP(FLAG/HA-NSP1-K164-H165A)
Plasmid#188782PurposeExpresses FLAG/HA-NSP1-K164-H165A in mammalian cells (SARS-CoV-2 mutant NSP1)DepositorInsertFLAG/HA-NSP1-K164-H165A (SARS-CoV-2)
UseFrt site to insert into hek293 t-rex genomeTagsFLAG/HAExpressionMammalianMutationChanged Lysine 164 to Alanine and Histidine 165 t…PromoterCMV Dox inducibleAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-SF14P
Plasmid#209448Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from SF14P promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-kasOP*
Plasmid#209447Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from kasOP* promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPKm-240
Plasmid#90510PurposepSIN - EF-1alpha - Cyto -sFd - P2A - Cyto - sFNR, vector encoding for cytoplasmic-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoterDepositorInsertcyto-sFD, cyto-sFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pPKm-300
Plasmid#104626PurposeExpresses pSIN - EF-1alpha - MTS - tFd, vector encoding mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd), under EF-1alpha promoterDepositorInserttFd
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1alphaAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastMutationPromoterTEF1pAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2
Plasmid#174491Purposebacterial expression of human SEPT2DepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RHO-IntC-dCas9C-VPR-polyA
Plasmid#166692PurposeExpress C-terminal part of split dCas9-VPRDepositorInsertIntC-dCas9C-VPR
UseAAV and CRISPRTagsExpressionMammalianMutationPromotershort human rhodopsin promoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX020
Plasmid#167146PurposeMoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamianaDepositorInsertfungal caffeoylpiruvate hydrolase, nnCPH
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN094
Plasmid#167155PurposeMoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plantsDepositorInsertp35s-npgA-ocsT
UseTagsExpressionPlantMutationPromoterp35sAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-TetR-neoR
Plasmid#153310PurposeTet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GWDepositorInsertTetR
UseTransposonTagsExpressionMutationPromoterSV40Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only