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pQE30-HypocratesCS

Plasmid

#161957

Purpose

Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

Depositor

Dmitry Bilan

Article

Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

Insert

HypocratesCS

Tags

6xHIs tag

Expression

Bacterial

Mutation

changed cysteine 355 to serine

Promoter

T5

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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D54_ Ld_061160

Plasmid

#135867

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 BICC FL

Plasmid

#112998

Purpose

For protein expression and purification of full-length Drosophila BicC

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length BicC (BicC Fly)

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

Plasmid

#111922

Purpose

Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_1.2μ

Tags

CoxVIIIx2

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_BZRAP1-2/3

Plasmid

#91551

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-2/3 (TSPOAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF2-2/3

Plasmid

#91557

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-2/3 (SH3RF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NOXO1-2/2

Plasmid

#91558

Purpose

Protein expression and purification of human SH3 domain construct NOXO1-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOXO1-2/2 (NOXO1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_BZRAP1-3/3

Plasmid

#91559

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-3/3 (TSPOAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRAP-2/2

Plasmid

#91529

Purpose

Protein expression and purification of human SH3 domain construct GRAP-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRAP-2/2 (GRAP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PIK3R2-1/1

Plasmid

#91535

Purpose

Protein expression and purification of human SH3 domain construct PIK3R2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PIK3R2-1/1 (PIK3R2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only