We narrowed to 11,803 results for: nsf

1. D70_Ld_210610

   Plasmid

   #135883

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   dihydrolipoamide acetyltransferase precursorlike protein
   

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. actc1b-GalT-mKate2

   Plasmid

   #109506

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT). Marks the Golgi complex.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-GalT-mKate2
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Thy1.1-muSOX D219A

   Plasmid

   #131704

   Purpose

   encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

   Depositor

   Britt Glaunsinger

   Article

   Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

   Insert

   Thy1.1-muSOX
   

   Expression

   Mammalian

   Mutation

   Aspartic acid 219 mutated to an alanine

   Promoter

   CMV

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-FLEX-mCherry-FHA1-subT391A-mCherry

   Plasmid

   #104462

   Purpose

   Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

   Insert

   mCherry-subT391A-FHA1-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   CAG

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMflT-o1

   Plasmid

   #101309

   Purpose

   Contains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Insert

   oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-Zeo-NKX2-8

   Plasmid

   #75090

   Purpose

   retrovirus-mediated gene transfer into mammalian cells

   Depositor

   David Mu

   Article

   Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Insert

   NKX2-8 (NKX2-8 Human)
   

   Use

   Retroviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild-type, full-length, without native 3' UTR

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCNL-N1_Golgi

   Plasmid

   #65694

   Purpose

   Expresses Golgi-CNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   N-terminal 81 amino acids of beta 1,4-galactosyltransferase
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYNL-N1_Golgi

   Plasmid

   #65693

   Purpose

   Expresses Golgi-YNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   N-terminal 81 amino acids of beta 1,4-galactosyltransferase
   

   Use

   Luciferase

   Tags

   YNL

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2 YFP-Smo-Weyer'sAla5

   Plasmid

   #41016

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   smoothened homolog (Smo Mouse)
   

   Tags

   EVC2 ala5 (aa 1177-1220 with amino acids 1185-118…

   Expression

   Mammalian

   Mutation

   YFP inserted after Smo signal sequence. Transfer…

   Available Since

   Nov. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVf-EnhCB-lacZnls mir-122 1xBS

    Plasmid

    #35643

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    1 miR-122 target site
    

    Use

    AAV

    Promoter

    CB

    Available Since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1-a and hU6

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKIIa-hChR2(H134R)-EYFP

    Plasmid

    #26969

    Purpose

    AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Histidine 134 is mutated to Arginine

    Promoter

    CaMKIIa

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. AV4-EF1-NMNAT2

    Plasmid

    #124970

    Purpose

    Express NMNAT2

    Depositor

    Kazuhiro Oka

    Article

    Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

    Insert

    NMNAT2, GFP (NMNAT2 Human)
    

    Use

    AAV

    Promoter

    EF1-alpha

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXWB-EBNA1

    Plasmid

    #37624

    Purpose

    Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-AncBE4max

    Plasmid

    #174164

    Purpose

    This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.

    Depositor

    Wensheng Wei

    Article

    Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

    Insert

    AncBE4max
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_OT1.0

    Plasmid

    #185386

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-LiLac

    Plasmid

    #184570

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    AAV

    Tags

    His7

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

    Plasmid

    #178960

    Purpose

    Expresses saCas9 protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Staphylococcus aureus Cas9 (SaCas9)
    

    Use

    AAV

    Tags

    3x HA

    Promoter

    gfaABC1D (astrocyte specific)

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-TRE-fDIO-GFP-IRES-tTA

    Plasmid

    #118026

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-fDIO-GFP-IRES-tTA
    

    Use

    AAV

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-Con/Fon oG

    Plasmid

    #131778

    Purpose

    INTRSECT 2.0

    Depositor

    Karl Deisseroth

    Article

    Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

    Insert

    rabies glycoprotein - oG
    

    Use

    AAV; Intrsect

    Tags

    none

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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