We narrowed to 23,621 results for: promoter

1. pcDNA3-FLAG-mTET2 (N1041)

   Plasmid

   #89737

   Purpose

   Expresses the N1041 truncation to mouse TET2 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-term 1041 truncation of mouse TET2 (contains fi…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

   Plasmid

   #163990

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1461R

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

   Plasmid

   #69049

   Purpose

   lentiviral expression of IKZF2 variant 2 with FLAG tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF2 (IKZF2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   splice variant 2

   Promoter

   CAG

   Available Since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

   Plasmid

   #187275

   Purpose

   Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Mutation

   deltaMyosin binding domain

   Promoter

   U6, CMV

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

   Plasmid

   #208944

   Purpose

   A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-FH-AGO2-R438E

   Plasmid

   #91984

   Purpose

   Expresses FLAG-HA-AGO2 (R438E)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   R438E

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Polymerase expression - pCMV-ePhi29 (LM2802)

   Plasmid

   #208967

   Purpose

   Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR - EGFP sgRNA 6

   Plasmid

   #51765

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 6
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EFS and U6

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCas9-sgCh2-2-Blast

   Plasmid

   #199645

   Purpose

   Expresses Cas9 and sgRNA control guide targeting intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX311 HRAS

    Plasmid

    #117749

    Purpose

    Open reading frame vector encoding HRAS

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    RASH1 (HRAS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Closed vector so will not read through the V5 reg…

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur

    Plasmid

    #69051

    Purpose

    lentiviral expression of IKZF1 with HA tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    UBC

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1-FH-AGO2-K525E

    Plasmid

    #91985

    Purpose

    Expresses FLAG-HA-AGO2 (K525E)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    K525E

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1 KBTBD4 P311PP

    Plasmid

    #184628

    Purpose

    Mammalian expression vector with N-terminal 3xFlag for KBTBD4 P311PP expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 P311PP (KBTBD4 Human)
    

    Expression

    Mammalian

    Mutation

    P311PP

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA 3.1 KBTBD4 R312RG

    Plasmid

    #184630

    Purpose

    Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)
    

    Expression

    Mammalian

    Mutation

    R312RG

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. px330-CIB1-dCas9

    Plasmid

    #63665

    Purpose

    Mammalian gRNA expression vector also expressing CIB1-dCas9

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and CMV

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid

    #208969

    Purpose

    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-ACSL4-FL

    Plasmid

    #202515

    Purpose

    Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    ACSL4 promoter (partial) (ACSL4 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human FLAG-WDR20

    Plasmid

    #217618

    Purpose

    Mammalian expression of FLAG tagged WDR20

    Depositor

    Ethan Lee

    Article

    Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

    Insert

    WDR20 (WDR20 Human)
    

    Expression

    Mammalian

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+_LN-HA-AGO2-WT

    Plasmid

    #92009

    Purpose

    Expresses Lambda-N-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

    Plasmid

    #208955

    Purpose

    A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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