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pLG-mLRRC45 (209 a.a.)

Plasmid

#240068

Purpose

ITT reporter plasmid for cold stress

Depositor

Hodaka Fujii

Article

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

LRRC45 C-terminal 209 a.a. (Lrrc45 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLG-mCCDC91 (198 a.a.)

Plasmid

#240069

Purpose

ITT reporter plasmid for cold stress

Depositor

Hodaka Fujii

Article

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

CCDC91 C-terminal 198 a.a. (Ccdc91 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9

Plasmid

#210036

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
puro-T2A-eGFP

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-EYFP

Plasmid

#231926

Purpose

Cre-on/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CG1358

Plasmid

#218541

Purpose

Drosophila CG1358 cDNA (human codon optimized)

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CG1358 (HisT Fly)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg2

Plasmid

#218531

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg1

Plasmid

#218530

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pKG-GW1-SRT1

Plasmid

#203171

Purpose

Yeast expression vector for yeast SRT1

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

SRT1 (SRT1 Budding Yeast)

Expression

Yeast

Promoter

TDH3

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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2x3APWWP_K299E_D333A-IFP2.0C

Plasmid

#215753

Purpose

Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2x3APWWP_K299E_D333A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCF702

Plasmid

#209650

Purpose

Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

Depositor

Bastian Molitor

Article

Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

Inserts

Downstream homologous region
thermostable neomycin phosphotransferase gene
Upstream homologous region

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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