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pFGC5941-PacI-ath-STTM171

Plasmid

#84098

Purpose

target miRNA171 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Publication

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM171

Expression

Plant

Promoter

2x35S

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM163

Plasmid

#84089

Purpose

target miRNA163 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Publication

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM163

Expression

Plant

Promoter

2x35S

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM162

Plasmid

#84088

Purpose

target miRNA162 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Publication

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM162

Expression

Plant

Promoter

2x35S

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE673

Plasmid

#153239

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_TET1cd

Plasmid

#106432

Purpose

pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Publication

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

pUBQ10::ZF108_3xFLAG_TET1cd (TET1 Synthetic, Human, Mustard Weed)

Use

Synthetic Biology

Expression

Plant

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM319/159

Plasmid

#84116

Purpose

target miRNA319/159 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Publication

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM319/159

Expression

Plant

Promoter

2x35S

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB602-OEP7-mVenus

Plasmid

#213864

Purpose

Binary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.

Depositor

Yutaka Kodama

Publication

Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

Insert

chloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)

Expression

Bacterial and Plant

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDGE77

Plasmid

#80579

Purpose

recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE79

Plasmid

#80581

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pILL5-NLS-3xmCitrine

Plasmid

#192525

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Publication

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

IAA-leu-resistant-like5 (ILL5 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

ILL5

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBT-6

Plasmid

#22829

Depositor

Ryan Gill

Publication

Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

Type

Empty backbone

Expression

Bacterial

Available since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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MTK3_002

Plasmid

#123719

Purpose

Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Blasticydin Resistance

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0586

Plasmid

#133473

Purpose

Integration into fission yeast genome at lys3 locus with bsdMX selection cassette

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBTB-6

Plasmid

#22822

Depositor

Ryan Gill

Publication

Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

Type

Empty backbone

Expression

Bacterial

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only

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pBMTL-1

Plasmid

#22811

Depositor

Ryan Gill

Publication

Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pGYL1_pPromALB_FireflyLuc

Plasmid

#220282

Purpose

Firefly luciferase with minimal Albumin promoter without enhancer; enhancer of interest can be cloned into MCS

Depositor

David Waxman

Publication

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Type

Empty backbone

Use

Luciferase

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEM8317

Plasmid

#213989

Purpose

PrpsM driven expression of sfGFP

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

sfGFP

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM10686

Plasmid

#213995

Purpose

PrpsM driven expression of mScarlet-I

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8313

Plasmid

#213986

Purpose

PrpsM driven expression of mCerulean

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8731

Plasmid

#213991

Purpose

PrpsM driven expression of mNeonGreen - sequence codon-optimized for Salmonella

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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