We narrowed to 14,442 results for: NTS

1. LV_EF1a_CCNB1-P2A-Hygro_Barcode

   Plasmid

   #170218

   Purpose

   Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CCNB1 (CCNB1 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFR G719S

   Plasmid

   #11013

   Purpose

   Retroviral construct for expressing human EGFR with G719S mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR G719S (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   G719S

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

3. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

   Plasmid

   #163030

   Purpose

   Expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 pA, and myc NLS

   Expression

   Mammalian

   Promoter

   Alb-AAT

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEMS-Tom20-Halo7Tag

   Plasmid

   #111135

   Purpose

   Tom20 with selflabeling tag, e.g. for fluorescence microscopy

   Depositor

   Karin Busch

   Article

   Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

   Insert

   Receptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
   

   Tags

   Halo7 Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiPGK Puro DEST JNKKTRmCerulean3

   Plasmid

   #90232

   Purpose

   Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. HiBiT-FZD1

   Plasmid

   #195839

   Purpose

   Expresses human FZD1 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD1 (FZD1 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-GST-DAXX-His

   Plasmid

   #175781

   Purpose

   DAXX protein for baculovirus expression system

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Tags

   GST and TEV

   Expression

   Insect

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCmini-iCAP-PHP.B3

   Plasmid

   #103004

   Purpose

   non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B3 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCmini-iCAP-PHP.B2

   Plasmid

   #103003

   Purpose

   non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B2 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   Dec. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 LIC 6A NLRP1-FLAG

    Plasmid

    #166803

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGM117

    Plasmid

    #220168

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    CISD1 (CISD1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19_CoV-2_linker

    Plasmid

    #211731

    Purpose

    Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

    Depositor

    Thomas Klimkait

    Article

    Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

    Insert

    Linker fragment SARS-CoV-2
    

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX4T2 RANK-L

    Plasmid

    #108571

    Purpose

    Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

    Depositor

    Jean-Claude Scimeca

    Article

    Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

    Insert

    RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    aa158-aa316

    Promoter

    Ptac (trp/lac)

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRES-hrGFP II-TET1_CD

    Plasmid

    #83570

    Purpose

    Expresses the catalytic domain of TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Mutation

    TET1 amino acids 1-1417 deleted

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

    Plasmid

    #160608

    Purpose

    TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    P35s:dCas9:SRDX:tNOS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28C-mCherry-HNRNPA1LCD

    Plasmid

    #204405

    Purpose

    E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    HNRNPA1 (186-320) (HNRNPA1 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-itis pBE-t

    Plasmid

    #195342

    Purpose

    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Inserts

    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    

    Use

    CRISPR

    Mutation

    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

    Promoter

    Lac and ProC

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hygro-iNGN3

    Plasmid

    #75340

    Purpose

    doxycycline inducible NGN3 gene over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    NGN3 (NEUROG3 Human)
    

    Expression

    Mammalian

    Promoter

    TRE-tight

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCPH2A.X_S139A-GS3

    Plasmid

    #204749

    Purpose

    Expression of Cas9 and human H2AX with the S139A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX S139A (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the S139A mutation

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFRT-DHX36iso2

    Plasmid

    #159586

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 2 in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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