We narrowed to 10,524 results for: SEC

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OA-1067A1

Plasmid

#164847

Purpose

Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

4 gRNAs and 3xP3-eGFP

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1067K

Plasmid

#164848

Purpose

Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

4 gRNAs and 3xP3-tdTomato

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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OA-874PA

Plasmid

#164846

Purpose

Expresses AAEL005635-Cas9 with OpIE2-CFP marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

P10-3'UTR OpIE2-CFP

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHPHS800

Plasmid

#228240

Purpose

Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Insert

Cas9

Tags

Hygromycin phosphotransferase

Expression

Mammalian

Promoter

none

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal4-3xP3-RFP

Plasmid

#127556

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal4

Use

CRISPR

Expression

Insect

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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SDHC-pHLuorin-sems

Plasmid

#107767

Purpose

labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit C of SDH fused to pHluorin (SDHC Human)

Tags

pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217515

Purpose

Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor Q63L

Plasmid

#12151

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor Q63L (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA Q63L (dominant-positive mutation)

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-5

Plasmid

#228965

Purpose

For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor T19N

Plasmid

#12152

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor T19N (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA T19N (dominant-negative mutation)*

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-3

Plasmid

#72345

Purpose

Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-hSpCas9

Plasmid

#162162

Purpose

Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

N/A

Expression

Insect

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)

Plasmid

#206461

Purpose

Oskar 139-240 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-240 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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IMPT-10316:GABR1

Plasmid

#157850

Purpose

HA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Article

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABR1 (GABBR1 Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-14

Plasmid

#228994

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-14

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(D323A/D325A)

Plasmid

#86592

Purpose

Expresses human full-length Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

D323A/D325A transforming mutations

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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GST-3C-FIP200-GFP

Plasmid

#187832

Purpose

Plasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

FIP200 (RB1CC1 Human)

Tags

GST and mGFP

Expression

Bacterial and Insect

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pFl Strep-Sumo-TEV human OAS1

Plasmid

#130895

Purpose

Human oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobos

Depositor

Leemor Joshua-Tor

Article

Ando et al Mol Cell. 2019 Feb 21;73(4):845-856.e5.

Insert

oligoadenylate synthase 1 (OAS1 Human)

Tags

Strep_Sumo

Expression

Insect

Promoter

ph

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0828 (pQCascade_crRNA-4)

Plasmid

#130637

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD5-U6-3-t-attP40

Plasmid

#133561

Purpose

gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

attP40 region guide RNAs

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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