We narrowed to 14,442 results for: NTS

1. p2.1 (pAGL2A)

   Plasmid

   #27563

   Depositor

   Gregg Semenza

   Article

   Semenza et al J Biol Chem. 1996 Dec 20. 271(51):32529-

   Insert

   ENO1 promoter (ENO1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

   Plasmid

   #233035

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and CasRx
   

   Use

   AAV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLM-mCitrine-Sox2

   Plasmid

   #23242

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

   Insert

   mCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)
   

   Use

   Lentiviral

   Available Since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. ITPKA-mTurquoise2

   Plasmid

   #137811

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   ITPKA (ITPKA Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-MBP-BrCas12b

   Plasmid

   #182276

   Purpose

   Expresses recombinant BrCas12b

   Depositor

   Piyush Jain

   Article

   Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, T7, Thrombin site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFR D837A

   Plasmid

   #11014

   Purpose

   Retroviral construct for expressing human EGFR with D837A mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR D837A (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   D837A

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pET-Duet1-His-TEV-mCherry-OPTN WT

   Plasmid

   #190191

   Purpose

   Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   optineurin (OPTN Human)
   

   Tags

   6xHis, TEV, and mCherry

   Expression

   Bacterial

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV-EF1aGFP2AKAT7(E508Q)-W

   Plasmid

   #159293

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 E508Q
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

   Promoter

   human EF1a promoter

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ-ABE-SpRYn-Act3.0

    Plasmid

    #178959

    Purpose

    It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ166-SpRY

    Plasmid

    #161520

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    zSpRYCas9
    

    Use

    CRISPR; Gateway compatible zsprycas9 entry clone

    Tags

    3X FLAG, NLS and NLS

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD_C2200

    Plasmid

    #91082

    Purpose

    Module C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    none (will be fused to gRNA array in MODULE B)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FSW-HRP-V5-LRRTM2

    Plasmid

    #82537

    Purpose

    Lentiviral vector for expressing HRP-LRRTM2 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    LRRTM2 (LRRTM2 Human)
    

    Use

    Lentiviral

    Tags

    HRP and V5

    Expression

    Mammalian

    Promoter

    Human Synapsin

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GB1-A11(89-196)

    Plasmid

    #196215

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(89-196).

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GB1-A11(2-68)-Strep

    Plasmid

    #196216

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-68)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GB1-A11(2-52)-Strep

    Plasmid

    #196213

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-52)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EFS-DjCas13d-HA-mCherry-pA

    Plasmid

    #233030

    Purpose

    To Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    DjCas13d-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1_T7-mScarlet3-Hras

    Plasmid

    #225928

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-Ras membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-HRas
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p420-3

    Plasmid

    #71257

    Purpose

    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

    Insert

    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF minimal

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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