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csd6_13-330_C176A/pET28b(+) N-tag

Plasmid

#71360

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Cysteine 176 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_H160A/pET28b(+) N-tag

Plasmid

#71359

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Histidine 160 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_W158A/pET28b(+) N-tag

Plasmid

#71358

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tryptophan 158 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_H155A/pET28b(+) N-tag

Plasmid

#71357

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:H155A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Histidine 155 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_Y132A/pET28b(+) N-tag

Plasmid

#71355

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:Y132A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tyrosine 132 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pJK347

Plasmid

#71833

Purpose

Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genes

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Inserts

hypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Expression

Bacterial

Mutation

changes histidine-59 to aspartate

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK348

Plasmid

#71832

Purpose

Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genes

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Inserts

hypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK174

Plasmid

#71835

Purpose

Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Expression

Bacterial

Mutation

additional N-terminal methionine encoded by this …

Promoter

T7 and endogenous

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTER SADB shRNA Human

Plasmid

#67151

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against human SADB

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (BRSK1 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1 Tet

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only
pUC19 gene 60 thru gap + Cys

Plasmid

#49161

Purpose

Template for generating gene 60 mRNA truncated two codons after coding gap + Cys

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Mutation

gene 60 mRNA truncated two codons after coding ga…

Promoter

T7

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcFNE3A (chick fibronectin E3A)

Plasmid

#14062

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin EIIIA (FN1 Chicken)

Expression

Mammalian

Mutation

Blunted BstE2-AvaII fragment was inserted into bl…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
hPrp16 C-terminus

Plasmid

#11205

Depositor

Robin Reed

Article

Zhou et al EMBO J. 1998 Apr 1. 17(7):2095-106.

Insert

Prp16 C-terminus (DHX38 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 948-1227

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pSL3 - Brr2 del113 N1104L

Plasmid

#90253

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 113 amino acids and amino acid N110…

Availability

Academic Institutions and Nonprofits only

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Pet28a His6-HSPB7 (zebrafish) WT

Plasmid

#108917

Purpose

Express WT Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Tags

His6

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pSL11 - Brr2 del422 N1104L

Plasmid

#90261

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid N110…

Availability

Academic Institutions and Nonprofits only

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pSL12 - Brr2 del422 Q904E

Plasmid

#90262

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid Q904E

Availability

Academic Institutions and Nonprofits only

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pSL13 - Brr2 del422 R1107L

Plasmid

#90263

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid R110…

Availability

Academic Institutions and Nonprofits only

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pAKW.119-R5-TEV-SUMO-His

Plasmid

#154050

Purpose

Expresses R5 peptide in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Silaffin R5 peptide

Tags

SUMO and His tag

Expression

Bacterial

Promoter

T5LacO

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-Pa01-bpNLS-3'UTR-polyA (CJT484)

Plasmid

#248218

Purpose

Pa01-bpNLS IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-Pa01-bpNLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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FRET-ON

Plasmid

#254591

Purpose

Engineered high-FRET standard for FRET ratio calibration

Depositor

Chuan-Hsiang Huang

Article

Wu et al iScience. 2025 Oct 11;28(11):113743. doi

Insert

FRET-ON

Use

Lentiviral

Tags

ECFP and YPet

Expression

Mammalian

Mutation

Y349F

Promoter

CMV

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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