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C-SWAT IAA17-6FLAG (no NLS)

Plasmid

#193322

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCEF-S15-FRB-Bla1

Plasmid

#190916

Purpose

Protein fragment complementation

Depositor

Jakob Reiser

Article

Ou et al Gene Ther. 2013 Jan;20(1):43-50. doi: 10

Insert

S15-FRB-Bla1 fusion protein

Tags

N-terminal fragment of BLA fused to c-Src and FRB…

Expression

Mammalian

Promoter

CEF

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProC

Plasmid

#198430

Purpose

mkate2 fluorescent reporter gene under control of promoter ProC. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProC and no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProD

Plasmid

#198431

Purpose

mkate2 fluorescent reporter gene under control of promoter ProD. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProD and no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-HsAsb9-3UTR_X

Plasmid

#147949

Purpose

Mammalian Expression of HsAsb9-3UTR

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsAsb9-3UTR (ASB9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos1-del1-39_X

Plasmid

#147932

Purpose

Mammalian Expression of HsNanos1-del1-39

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-del1-39 (NANOS1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos1-del1-58_X

Plasmid

#147933

Purpose

Mammalian Expression of HsNanos1-del1-58

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-del1-58 (NANOS1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos1-del40-56_X

Plasmid

#147934

Purpose

Mammalian Expression of HsNanos1-del40-56

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-del40-56 (NANOS1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-CeSmg5_S

Plasmid

#147456

Purpose

Mammalian Expression of CeSmg5

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5 (smg-5 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-CeSmg5-G91E_S

Plasmid

#147457

Purpose

Mammalian Expression of CeSmg5-G91E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5-G91E (smg-5 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-CeSmg5-Q94E_S

Plasmid

#147459

Purpose

Mammalian Expression of CeSmg5-Q94E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5-Q94E (smg-5 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-CeSmg7_S

Plasmid

#147465

Purpose

Mammalian Expression of CeSmg7

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7 (smg-7 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-CeSmg7-G80E_S

Plasmid

#147466

Purpose

Mammalian Expression of CeSmg7-G80E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7-G80E (smg-7 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-CeSmg7-T83E_S

Plasmid

#147467

Purpose

Mammalian Expression of CeSmg7-T83E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7-T83E (smg-7 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-CeSMG5_1-420_S

Plasmid

#147468

Purpose

Bacterial Expression of CeSMG5_1-420

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSMG5_1-420 (smg-5 Nematode)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-CeSMG5_1-420-G91E_S

Plasmid

#147469

Purpose

Bacterial Expression of CeSMG5_1-420-G91E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSMG5_1-420-G91E (smg-5 Nematode)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetMP-CeSmg7_1-395_S

Plasmid

#147475

Purpose

Bacterial Expression of CeSmg7_1-395

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7_1-395 (smg-7 Nematode)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-Q123E_T

Plasmid

#147555

Purpose

Mammalian Expression of HsSmg5-Q123E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-Q123E (SMG5 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-dsRNAres_N

Plasmid

#147029

Purpose

Insect Expression of DmNot1-dsRNAres

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmNot1-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-417-4Ala-mut-V5His6_M

Plasmid

#146897

Purpose

Insect Expression of DmCup_1-417-4Ala-mut

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-417-4Ala-mut (cup Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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