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T7-5'UTR-IS621-NLS-3'UTR-polyA (CJT503)

Plasmid

#248221

Purpose

IS621-bpNLS IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-IS621-NLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-bpNLS-BceINT-3'UTR-polyA (CJT483)

Plasmid

#248217

Purpose

bpNLS-BceINT IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-bpNLS-BceINT-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-bpNLS-SacINT-3'UTR-polyA (CJT485)

Plasmid

#248219

Purpose

bpNLS-SacINT IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-bpNLS-SacINT-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD726

Plasmid

#253049

Purpose

Encodes OST1 Sig Pep, LC Cutinase-ICCG variant, FLAG-tagged SED1 surface display anchor in a yeast expression vector with pTEF1 promoter, tTDH1 term, LEU2 selectable marker and CEN/ARS origin

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Leaf compost cutinase

Expression

Yeast

Mutation

Y127G, D238C, F243I, S283C

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD310

Plasmid

#253032

Purpose

Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Anti-GFP nanobody 649-stalk anchor

Expression

Yeast

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(mono)

Plasmid

#254354

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

Duplicate residues 222-273 within 228-229, flanke…

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag

Plasmid

#246867

Purpose

Express HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag (M2-1exo-SunTag) for RSV imaging.

Depositor

Marvin Tanenbaum

Article

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

The RSV M2-1 protein with a C-terminal insertion …

Promoter

SFFV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-Myc-TRIP12-SB-Hygro

Plasmid

#216772

Purpose

Myc-TRIP12-SB; the substrate binding (SB) domain (AA 1-1650) of TRIP12 with an N-terminal Myc-tag & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Insert

Myc-TRIP12-SB (TRIP12 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR-Flag-Polb(TM)-C

Plasmid

#216775

Purpose

Flag-Polb(TM), C-terminal domain (AA 91-335); TM = L301R/V303R/V306R

Depositor

Robert Sobol

Article

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Insert

Flag-Polb (POLB Human)

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain (amino acids 91-335); TM = L301…

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pX330_mouseH3.1

Plasmid

#244241

Purpose

Expresses SpCas9 and a sgRNA targeting the mouse histone H3.1 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

sgRNA targeting mouse histone H3.1 (H3c1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1_N300

Plasmid

#228814

Purpose

Expression of human EHMT1 N-terminal 300 aa only

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains aa 1-300 only

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K519TAG)-HA

Plasmid

#239782

Purpose

Expresses rat NF186 with a TAG codon at position 519 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K519TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K534TAG)-HA

Plasmid

#239783

Purpose

Expresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K534TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K571TAG)-HA

Plasmid

#239784

Purpose

Expresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K571TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K604TAG)-HA

Plasmid

#239785

Purpose

Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K604TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K680TAG)-HA

Plasmid

#239786

Purpose

Expresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K680TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K809TAG)-HA

Plasmid

#239787

Purpose

Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K809TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C F87E

Plasmid

#238074

Purpose

Bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S S85C F87E

Promoter

T7

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, K98A)

Plasmid

#231377

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

H56A, K98A

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A, K98A)

Plasmid

#231378

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R97A, K98A

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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