Addgene

We narrowed to 25,797 results for: sta

Showing: 8161 - 8180 of 25797 results

Results per page:

Sort results by:

pTRANS_223

Plasmid

#91117

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Use

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-RABIF-FLAG

Plasmid

#135558

Purpose

Express human RABIF in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

RABIF (RABIF Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

T7 promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_C3010

Plasmid

#91098

Purpose

Module C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Tags

Expression

Mutation

Promoter

ZmUbi

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO/intron/GFP

Plasmid

#113547

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-B41gp160

Plasmid

#123247

Purpose

Mammalian expression plasmid for Env from the B41 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (B41) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTarget

Plasmid

#186452

Purpose

used as source for acquisition of new spacers

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
px458_2A_GFP_sgRNA_ZNF598

Plasmid

#127121

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA ZNF598 (ZNF598 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pX335-NEAT1_IS_v2

Plasmid

#97086

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v2

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
P2A-NLS-tdtomato-EPG backbone

Plasmid

#215548

Purpose

An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC5

Plasmid

#104779

Purpose

pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-CD9-10

Plasmid

#57356

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD9 (CD9 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pvimentin-miRFP713

Plasmid

#136569

Purpose

Vimentin labeling with near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-miRFP713

Use

Tags

miRFP713

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SPI1_pet28a

Plasmid

#131653

Purpose

Bacterial expression of ETS gene SPI1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPI1 (SPI1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE:CloverCP-EFS:ZeoR

Plasmid

#68478

Purpose

Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

CloverCP
Zeocin Resistance

Use

Lentiviral

Tags

"CP" degradation domain

Expression

Mammalian

Mutation

Promoter

EFS and pTRE

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB035h

Plasmid

#79191

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-HA4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR221-mCherry

Plasmid

#79509

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

mCherry RFP

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(+24)

Plasmid

#199163

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+24)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
RP1B-PP1alpha

Plasmid

#51768

Purpose

Expresses PP1 alpha residues 7-330

Depositor

Wolfgang Peti

Article

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

pp1 alpha

Use

Tags

His, TEV protease site, and Thio-6

Expression

Bacterial

Mutation

Contains PP1 alpha residues 7-330

Promoter

T7-lac

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

Plasmid

#172225

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge