Addgene

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pGEX4T1_GST-EGFP-ATG13_IDR (191-517aa)

Plasmid

#223760

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 IDR (ATG13 Human)

Use

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 191-517 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-TEV-EGFP-ATG13_IDR_191-517aa_(M196A_S197A_R199A)

Plasmid

#223761

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 IDR (ATG13 Human)

Use

Tags

GST-TEV-EGFP

Expression

Bacterial

Mutation

expresses aa 191-517, with M196A S197A R199A (par…

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

Plasmid

#223762

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 IDR (ATG13 Human)

Use

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

Plasmid

#223765

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BNIP3 soluble part (BNIP3 Human)

Use

Tags

EGFP-TEV-GST

Expression

Insect

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)

Plasmid

#223766

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BNIP3 soluble part (BNIP3 Human)

Use

Tags

EGFP-TEV-GST

Expression

Insect

Mutation

W18A/L21A; deletion of LIR

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (WT)

Plasmid

#223736

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Use

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (WT)

Plasmid

#223737

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Use

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)

Plasmid

#223738

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

W36A/L39A; deletion of LIR

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR)

Plasmid

#223739

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

FUNDC1 soluble part, mutant (FUNDC1 Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

Y18A/L21A; deletion of LIR

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-TEV-SRC (Y530F)

Plasmid

#223743

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

Src Kinase (SRC Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Y530F constitutively active mutant

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST

Plasmid

#223744

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BCL2L13 soluble part (BCL2L13 Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST

Plasmid

#223745

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BCL2L13 soluble part (BCL2L13 Human)

Use

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (W36A/L39A; deltaLIR)

Plasmid

#223748

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Use

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

W36A/L39A; deletion of LIR

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-LC3A

Plasmid

#223726

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

LC3A (MAP1LC3A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-120 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-GBRPL1

Plasmid

#223729

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

GABARAPL1 (GABARAPL1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-GBRPL2

Plasmid

#223730

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet1_NIX(1-182aa)-THROMBIN-GST (WT)

Plasmid

#223733

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

aa 1-182 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (WT)

Plasmid

#223734

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

FUNDC1 soluble part (FUNDC1 Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

aa 1-50 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-GP78

Plasmid

#231689

Purpose

Expresses human GP78 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

GP78 (AMFR Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET28:LanIV-Chondromyces

Plasmid

#225078

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Chondromyces)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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