Addgene

We narrowed to 5,865 results for: pcDNA 3.1

Showing: 1201 - 1230 of 5865 results

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pcDNA3.1-Flag-stm2585_Y167F

Plasmid

#174392

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Use

Tags

Flag

Expression

Mammalian

Mutation

Tyr 167 changed to Phe

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585

Plasmid

#174391

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-6His-mRplp1

Plasmid

#173153

Purpose

mouse Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) expression plasmid in mammalian cells

Depositor

Brijesh Kumar Singh

Article

Tripathi et al bioRxiv 2021.11.16.468891

Insert

ORF clone Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) (Rplp1 Mouse)

Use

Tags

His

Expression

Mammalian

Mutation

Wild type

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11A

Plasmid

#159131

Purpose

Expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Use

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Y11A

Promoter

CMV

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) eGFP SH3BP5 G4

Plasmid

#129022

Purpose

Expresses an eGFP mRNA with the SH3BP5 G-quadruplex (G4) region in the 3' UTR

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1beta

Plasmid

#124140

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HF2-BE3

Plasmid

#120397

Purpose

Expresses HF2-BE3 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE3

Use

Tags

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_6367-EYFP

Plasmid

#111080

Purpose

Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin R1ACR_6367

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RlACR_497-EYFP

Plasmid

#111078

Purpose

Anion channelrhodopsin from Rhodomonas lens for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RlACR_497

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PsuACR_353-EYFP

Plasmid

#111076

Purpose

Anion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin PsuACR_353

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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W250R EPHA3 pcDNA3.1

Plasmid

#102744

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

W250R (TGG > AGG)

Promoter

CMV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.S61P

Plasmid

#105048

Purpose

Overexpression of APOBEC3C S61P mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

serine 61 to proline

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.D99E

Plasmid

#105049

Purpose

Overexpression of APOBEC3C D99E mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Aspartate 99 to glutamate

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.DS-EP

Plasmid

#105050

Purpose

Overexpression of APOBEC3C DS-EP mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

S61P, D99E

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.C97S

Plasmid

#105051

Purpose

Overexpression of APOBEC3C C97S mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

cysteine 97 to serine

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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D678E EPHA3 pcDNA3.1

Plasmid

#102750

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

D678E (GAC > GAa)

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_877-EYFP

Plasmid

#103883

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_877

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-R1ACR_447-EYFP

Plasmid

#103960

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_447

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-R2ACR_853-EYFP

Plasmid

#103963

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R2ACR_853

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-C1ACR_023-EYFP

Plasmid

#103966

Purpose

Anion channelrhodopsin from unclassified cryptophyte (CCMP2293) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin C1ACR_023

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-R1ACR_799-EYFP

Plasmid

#103962

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_799

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-C1ACR_887-EYFP

Plasmid

#103965

Purpose

Anion channelrhodopsin from unclassified cryptophyte (CCMP2293) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin C1ACR_887

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-PsuACR_433-EYFP

Plasmid

#103529

Purpose

Anion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin PsuACR_433

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-G1ACR_203-EYFP

Plasmid

#103143

Purpose

Anion channelrhodopsin from Geminigera sp. (Caron Lab Isolate) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin G1ACR_203

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-GcACR_145-EYFP

Plasmid

#103138

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_145

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-R1ACR_367-EYFP

Plasmid

#103773

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_367

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-GcACR_439-EYFP

Plasmid

#103142

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_439

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
T660K EPHA3 pcDNA3.1

Plasmid

#102749

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

T660K (ACA > AAA)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
S449F EPHA3 pcDNA3.1

Plasmid

#102748

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

S449F (TCC > TTC)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
A435S EPHA3 pcDNA3.1

Plasmid

#102747

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

A435S (GCT > TCT)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge