Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,360 results for: 256

Showing: 1601 - 1640 of 4360 results

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GPR89B_full_pGCS1

Plasmid

#238499

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

GPR89B (GPR89B Human)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MERS-CoV spike all7KO mutant

Plasmid

#231924

Purpose

For expression of MERS-CoV spike protein with glycan deletion at N1176, N1213, N1225, N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike all7KO (S MERS-CoV)

Expression

Mammalian

Mutation

N1176Q, N1213Q, N1225Q, N1241Q, N1256Q, N1277Q, N…

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo24

Plasmid

#203994

Purpose

Cloning plasmid containing the Hro ES5 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES5

Expression

Plant

Promoter

Hro ES5

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-FCPF

Plasmid

#220132

Purpose

Expresses Cas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

Cas9-FCPF (cas9 S. pyogenes)

Tags

His and NLS

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-FCPF

Plasmid

#220131

Purpose

Expresses dCas9-FCPF in mammalian cells

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 Synthetic, S. pyogenes)

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET-dCas9-FCPF

Plasmid

#220133

Purpose

Expresses dCas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 Synthetic, S. pyogenes)

Tags

6xHis

Expression

Bacterial

Mutation

FCPF sequence inserted on C-terminal

Promoter

T7

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225062

Purpose

Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

Plasmid

#225061

Purpose

Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225063

Purpose

Expression of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(158-238)

Plasmid

#220744

Purpose

GAL4-sfGFP(158-238) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(158-238)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNLS-VP16-NIR-Fb(718)

Plasmid

#220745

Purpose

NLS-VP16-NIR-Fb(718) consrtuct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-Fb(718)

Use

Affinity Reagent/ Antibody

Tags

NLS-VP16

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-NIR-FbLAG30

Plasmid

#220746

Purpose

GAL4-NIR-FbLAG30 construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-NIR-FbLAG30

Plasmid

#220748

Purpose

NIR-FbLAG30 expression in bacteria.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Expression

Bacterial

Mutation

For bacterial cytoplasmic expression, the pelB si…

Promoter

T7

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG16

Plasmid

#220749

Purpose

NIR-FbLAG16 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG16

Use

AAV and Affinity Reagent/ Antibody

Promoter

CAG

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG30

Plasmid

#220750

Purpose

NIR-FbLAG30 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

AAV and Affinity Reagent/ Antibody

Promoter

CAG

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-3xFLAG-SMAD1-active

Plasmid

#222579

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD1 (SMAD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

S487E, S489E

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9-FCPF

Plasmid

#220141

Purpose

Expresses Cas9-FCPF in mammalian cells

Depositor

Xinlai Cheng

Article

Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

Insert

Cas9-FCPF (cas9 Synthetic, S. Pyogenes)

Tags

Flag

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo25

Plasmid

#203995

Purpose

Cloning plasmid containing the Hro ES6 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES6

Expression

Plant

Promoter

Hro ES6

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo23

Plasmid

#203993

Purpose

Cloning plasmid containing the Hro ES4 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES4

Expression

Plant

Promoter

Hro ES4

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo32

Plasmid

#204006

Purpose

Cloning plasmid containing the Bpi ES7 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Bpi ES7

Expression

Plant

Promoter

Bpi ES7

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo33

Plasmid

#204007

Purpose

Cloning plasmid containing the Bpi ES8 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Bpi ES8

Expression

Plant

Promoter

Bpi ES8

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo34

Plasmid

#204008

Purpose

Cloning plasmid containing the Sma ES1 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Sma ES1

Expression

Plant

Promoter

Sma ES1

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo35

Plasmid

#204009

Purpose

Cloning plasmid containing the Sma ES2 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Sma ES2

Expression

Plant

Promoter

Sma ES2

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo36

Plasmid

#204010

Purpose

Cloning plasmid containing the Sma ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Sma ES3

Expression

Plant

Promoter

Sma ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo37

Plasmid

#204012

Purpose

Cloning plasmid containing the Sma ES4 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Sma ES4

Expression

Plant

Promoter

Sma ES4

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo38

Plasmid

#204013

Purpose

Cloning plasmid containing the Sma ES5 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Sma ES5

Expression

Plant

Promoter

Sma ES5

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo39

Plasmid

#204014

Purpose

Cloning plasmid containing the sfGFP insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

super folder green fluorescent protein

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo4

Plasmid

#203925

Purpose

Cloning plasmid containing the R6Kγ origin and oriT insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

R6Kγ origin of replication and oriT

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo11

Plasmid

#203981

Purpose

Cloning plasmid containing the Lambda tl3 terminator insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Lambda tl3 Terminator

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo13

Plasmid

#203983

Purpose

Cloning plasmid containing the tonB & SoxR terminators insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

tonB & SoxR Terminator

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo14

Plasmid

#203984

Purpose

Cloning plasmid containing the Elu ES1 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES1

Expression

Plant

Promoter

Elu ES1

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo15

Plasmid

#203985

Purpose

Cloning plasmid containing the Elu ES2 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES2

Expression

Plant

Promoter

Elu ES2

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo16

Plasmid

#203986

Purpose

Cloning plasmid containing the Elu ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES3

Expression

Plant

Promoter

Elu ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo18

Plasmid

#203988

Purpose

Cloning plasmid containing the Ppu ES2 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ppu ES2

Expression

Plant

Promoter

Ppu ES2

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo19

Plasmid

#203989

Purpose

Cloning plasmid containing the Ppu ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ppu ES3

Expression

Plant

Promoter

Ppu ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo22

Plasmid

#203992

Purpose

Cloning plasmid containing the Hro ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES3

Expression

Plant

Promoter

Hro ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo26

Plasmid

#204000

Purpose

Cloning plasmid containing the Bpi ES1 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Bpi ES1

Expression

Plant

Promoter

Bpi ES1

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo28

Plasmid

#204002

Purpose

Cloning plasmid containing the Bpi ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Bpi ES3

Expression

Plant

Promoter

Bpi ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRMTK-clo1

Plasmid

#203919

Purpose

Cloning plasmid containing the BHR1 origin insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

BHR1 Origin of Replication

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOAD eIF4G1 (1-341)

Plasmid

#196833

Purpose

Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.

Depositor

Stan Fields

Article

Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

Insert

eIF4G1 (TIF4631 Budding Yeast)

Tags

Gal4

Expression

Yeast

Mutation

amino acids 1-341

Promoter

ADH

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1601 - 1640 of 4360 results

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