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Plasmid#236527PurposeFor expression of ZO-1 (human) in insect cellsDepositorInsertTJP1 (TJP1 Human)
UseTagsSPY Tag and StrepTag-Tev-AviTagExpressionInsectMutationWTPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pNDGG049
Plasmid#231318PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. -pBBR1-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG011, pOGG012, and pOGG014, GmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG050
Plasmid#231319PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1a ActBT2ATetP2AP - mAGH
Plasmid#229973PurposeHarbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-HygroDepositorInsertActBT2ATetP2AP - mAGH
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(1-400)
Plasmid#228830PurposeInsect cell expression of N-terminal portion of BICD2 (aa 1-400)DepositorInsertBICD2 (Bicd2 Mouse)
UseTagsStrepExpressionInsectMutationaa 1-400PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(1-710)
Plasmid#228831PurposeInsect cell expression of truncated BICD2 (aa 1-710)DepositorInsertBICD2 (Bicd2 Mouse)
UseTagsStrepExpressionInsectMutationaa 1-710PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE WT SOX pACEmam1
Plasmid#192049PurposeExpresses WT SOX (pACEmam1)DepositorInsertWT SOX
UseUnspecifiedTagsExpressionMutationPromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-ppiB
Plasmid#202482PurposeExpresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletionDepositorInsertpeptidyl-prolyl cis-trans isomerase (ppiB)
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT011
Plasmid#225162PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKanB
Plasmid#223523PurposeUsed for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAOX1Available sinceSept. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHBJT012
Plasmid#225163PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-dCas9
Plasmid#220193Purposemini-Tn7 dCas9 expression vectorDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-GFP
Plasmid#220197Purposemini-Tn7 GFP expression vectorDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAceBAC2-MLG-HMF-LST2
Plasmid#220787PurposeExpresses N-terminal His10-Myc-FLAG-LST2 in insect cellsDepositorInsertLST2 (ZFYVE28 Human)
UseTags10xHis-Myc-FLAGExpressionInsectMutationPromoterp10Available sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN667
Plasmid#219963PurposeExpressed sfGFP fused human KIF1Bß(1-721) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
UseTagssfGFP::2xStrepII tagExpressionInsectMutationencodes 1-721PromoterpolyhedrinAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN668
Plasmid#219962PurposeExpressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
UseTagssfGFP::2xStrepII tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only