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Plasmid#129317Purposemutant of QUEEN-7+D20DepositorInsertQUEEN-7mu R122A
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG-altTAG
Plasmid#158146PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-tglA(C51)
Plasmid#134674PurposeExpresses His6-TglA-Cys in E. ColiDepositorInsertTglA(C51)
UseTagsHis6ExpressionBacterialMutationinsertion of cysteine 51PromoterT7Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(G:H)
Plasmid#138257PurposeExpression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter systemDepositorInsertGFP targeting sgRNAs G and H
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET_His-rsCaMPARI-mRuby3
Plasmid#120804PurposeExpresses rsCaMPARI in bacterial cellsDepositorInsertrsCaMPARI
UseTagsHis-Xpress and mRuby3ExpressionBacterialMutationPromoterT7Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
brr2-C520R pRS313
Plasmid#111412Purposebrr2-C520R (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationC520R aa mutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
brr2-N1104A pRS313
Plasmid#111414Purposebrr2-N1104A (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationN1104A aa mutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107L pRS313
Plasmid#111416Purposebrr2-R1107L (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationR1107L aa mutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315-cwc25 (aa 26-33 deleted)
Plasmid#111346PurposeCwc25 in pRS315(Leu) vectorDepositorInsertCWC25
UseTagsnoneExpressionYeastMutationaa 26-33 deletedPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315-cwc25 (aa 26-29 deleted)
Plasmid#111345PurposeCwc25 in pRS315(Leu) vectorDepositorInsertCWC25
UseTagsnoneExpressionYeastMutationaa 26-29 deletedPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315-cwc25 (aa 26-27 deleted)
Plasmid#111344PurposeCwc25 in pRS315(Leu) vectorDepositorInsertCWC25
UseTagsnoneExpressionYeastMutationaa 26-27 deletedPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 snu114-del(IV)
Plasmid#111321Purposesnu114-del(IV)DepositorInsertSNU114
UseTagsExpressionYeastMutationdeletion of domain IVaPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 myc-SNU114
Plasmid#111317Purposemyc-SNU114DepositorInsertSNU114
UseTagsmycExpressionYeastMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 snu114(G145A)
Plasmid#111319Purposesnu114(G145A)DepositorInsertSNU114
UseTagsExpressionYeastMutationsnu114 G145APromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 AAAA
Plasmid#87600PurposeEncodes Prp5 AAAA for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationD136A, P137A, L138A, D139APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 E235A
Plasmid#87601PurposeEncodes Prp5 E235A for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationE235APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 N399D
Plasmid#87602PurposeEncodes Prp5 N399D for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN399DPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 TAG
Plasmid#87603PurposeEncodes Prp5 SAT motif to TAG for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationS446T, T448GPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS315-PGK-SEIPIN2
Plasmid#96987PurposeExpress GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.DepositorInsertSEIPIN2 (AT1G29760 Mustard Weed)
UseTagsExpressionYeastMutationPromoterPhosphoglycerate Kinase (PGK) promoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 P369E
Plasmid#87277PurposeEncodes Hsh155 P369E for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationP369EPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only