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pBAD_SynPspA

Plasmid

#223814

Purpose

Bacterial expression of SynPspA with C-terminal YFP

Depositor

Carsten Sachse

Article

Junglas et al Cell. 2021 Jul 8;184(14):3674-3688.e18.

Insert

SynPspA

Tags

YFP

Expression

Bacterial

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET50b_SynPspA

Plasmid

#223813

Purpose

Bacterial expression of SynPspA with N-terminal NusA-tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Cell. 2021 Jul 8;184(14):3674-3688.e18.

Insert

SynPspA

Tags

NusA

Expression

Bacterial

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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PTGR-PS2

Plasmid

#237442

Purpose

Expressing PS2 S-layer from C. glutamicum under its native promoter

Depositor

Han Remaut

Article

Sogues et al Proc. Natl. Acad. Sci. U.S.A. 122 (31) e

Insert

cpsB

Expression

Bacterial

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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p365_DG

Plasmid

#234688

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and ALFA

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

ALFA and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGGC018

Plasmid

#228232

Purpose

Level 0 for LhG4 (GreenGate C module)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

GR-LhG4

Use

Synthetic Biology

Promoter

-

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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8_T7-PH-mScarlet3

Plasmid

#225935

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

PH-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAG369

Plasmid

#226748

Purpose

CRISPRi guide RNA against EMC2

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

EMC2 (EMC2 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_Avi-3C-TS

Plasmid

#223894

Purpose

4G-cloning donor for C-terminal AviTag-3C-TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

AviTag-3C-TwinStrep

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6

Plasmid

#223871

Purpose

4G-cloning donor for N-terminal His6 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6

Use

Golden-gate donor

Tags

6xHis

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_T7N

Plasmid

#223855

Purpose

4G-cloning donor for bacterial promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_T7C

Plasmid

#223856

Purpose

4G-cloning donor for bacterial promoter and terminator, C-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_T7

Plasmid

#223854

Purpose

4G-cloning donor for bacterial promoter and terminator, no tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His10-TS-3C

Plasmid

#223884

Purpose

4G-cloning donor for N-terminal His10-TwinStrep-3C tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His10-TwinStrep-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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AA018

Plasmid

#215976

Purpose

Fragmid fragment: (N' terminus) 4xVP16 transcritional activators

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; VP64_v1.1

Use

Fragment

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA134

Plasmid

#216044

Purpose

Fragmid fragment: (C' terminus) SV40NLS; Linker; MeCP2

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.7; Linker_v4.1; MeCP2_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA266

Plasmid

#216064

Purpose

Fragmid fragment: (2A Selection) TagRFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; TagRFP_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA267

Plasmid

#216065

Purpose

Fragmid fragment: (2A Selection) TagRFP657

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; TagRFP657_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA200

Plasmid

#216051

Purpose

Fragmid fragment: (C' terminus) p65; HSF1; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

p65_v1.2; HSF1_v1.1; NpNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA414

Plasmid

#215991

Purpose

Fragmid fragment: (N' terminus) conditional degradation

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; V5_v1.1; HaloTag_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA175

Plasmid

#216062

Purpose

Fragmid fragment: (2A Selection) EGFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; EGFP_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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