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pLenti-TRE-PtenWT-Blast

Plasmid

#135674

Purpose

Dox-inducible expression (TRE promoter) of wildtype Pten cDNA, Blasticidin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P**-R

Plasmid

#189851

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P**-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P** (modified T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-cat-P-R

Plasmid

#189853

Purpose

Cre-inducible activation of cat for chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-cat

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pNJB80

Plasmid

#51518

Purpose

Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Gateway entry vector

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGCS-C3m(3xFLAG)

Plasmid

#85726

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xFLAG

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP

Plasmid

#134357

Purpose

Reporter plasmid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Inserts

GFP
mCherry

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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p7XNH3

Plasmid

#47064

Purpose

FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBbS5c-Maqu_3494

Plasmid

#86205

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Expression

Bacterial

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-131

Plasmid

#185923

Purpose

Himar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mTurquoise2

Expression

Bacterial

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pZE31-DsRed-ms2x2

Plasmid

#26248

Depositor

Philippe Cluzel

Article

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

ms2 binding sites (x2)

Expression

Bacterial

Available Since

Oct. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pGGF000

Plasmid

#48861

Purpose

Empty entry vector for creating GreenGate plant resistance cassette modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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BBa_J72113-BBa_J72152

Plasmid

#40784

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

Phagemid + GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pBPGAL80Uw-4

Plasmid

#26235

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL80 (GAL80 Budding Yeast)

Expression

Insect

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pGCS-C2m(3xHA)

Plasmid

#85725

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-ccdB chr I

Plasmid

#41894

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Expression

Yeast

Promoter

GPD

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pUPD2-HR1b-C (B3-B4)

Plasmid

#211851

Purpose

CDS for HR1b degron sequence in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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LSL

Plasmid

#66590

Purpose

Lox-STOP-Lox reporter

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL2801

Plasmid

#71207

Purpose

AD1 Sense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

AD1 Sense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Available Since

Jan. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJJH1320

Plasmid

#36915

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

Cre recombinase

Expression

Bacterial

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pDO23A

Plasmid

#27367

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

pUC/ori, bla

Use

Gateway donor vector

Expression

Bacterial

Mutation

N/A

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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