We narrowed to 11,712 results for: NSI

1. pDONR223-ZNF232

   Plasmid

   #88305

   Purpose

   Donor Vector containing ZNF232 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF232 (ZNF232 Human)
   

   Use

   Gateway donor vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TVBB C-term-Dendra2-Puro

   Plasmid

   #169217

   Purpose

   Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked Dendra2-P2A-Puro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET41s-GST-ATF6a-AD

   Plasmid

   #64774

   Purpose

   bacterial expression of GST-tagged ATF6 (1-150)

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   ATF6a-AD (1-150) (ATF6 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-term deletion

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDONR221-ZNF442

   Plasmid

   #101455

   Purpose

   Donor Vector containing ZNF442 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF442 (ZNF442 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNOC_hfnCas12a-Nlux_crRNA NR1

   Plasmid

   #176244

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Promoter

   Ribosomal subunit

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223-ZNF366

   Plasmid

   #88019

   Purpose

   Donor Vector containing ZNF366 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF366 (ZNF366 Human)
   

   Use

   Gateway donor vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBMN-neo-Flag-TNFR1 L380A Del229-244

   Plasmid

   #44098

   Depositor

   Martin Kluger

   Article

   D'Alessio et al J Biol Chem. 2010 Jul 30;285(31):23868-7

   Insert

   human tumor necrosis factor receptor 1 (TNFRSF1A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Mutation

   L380A

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pDONR223-ZNF175

   Plasmid

   #88551

   Purpose

   Donor Vector containing ZNF175 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF175 (ZNF175 Human)
   

   Use

   Gateway donor vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGAL4-DBD-HOXA13-58A-IDR

   Plasmid

   #145238

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXA13-58A IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13-58A-IDR (HOXA13 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-Flag-Med25-VWA

    Plasmid

    #64772

    Purpose

    Mammalian expression of Flag-tagged Med25-VWA

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VWA (MED25 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA8-Flag-LARS(1-360aa)

    Plasmid

    #139668

    Purpose

    Expresses N-teminal Flag tagged aa 1-360 of LARS1 protein

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    Leucyl-tRNA Synthetase (LARS1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ239-RVR

    Plasmid

    #108863

    Purpose

    FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N623R, K629R and N633R, Cpf1 is rice codon optimi…

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3xFLAG CMV14 / BRAF-G466V

    Plasmid

    #131722

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    G466V

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-321/+120 Ebox mut1

    Plasmid

    #35544

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    T to G at position -96 in promoter

    Promoter

    none

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-321/+120 Ebox mut2

    Plasmid

    #35545

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    T to G at position -52 in promoter

    Promoter

    none

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCR3-vsv-AuroraB L154G/H250Y

    Plasmid

    #108490

    Purpose

    expression of vsv-AuroraB Analog sensitive (L154G/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Analog sensitive L154G/H250Y

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Desmoplakin II no force control (FL-based)

    Plasmid

    #118724

    Purpose

    The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-FL-mCherry

    Expression

    Mammalian

    Mutation

    truncation after aa1353

    Promoter

    CMV

    Available Since

    Dec. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-zeo: alpha6mGFP

    Plasmid

    #35611

    Purpose

    mammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP tag

    Depositor

    Henry Lester

    Article

    Xiao et al Biochem Pharmacol. 2011 Oct 15;82(8):852

    Insert

    nAChr alpha6mEGFP (Chrna6 Mouse)
    

    Tags

    mEGFP inserted after A405 with AGA linker on eith…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLL6 sgRNA(MS2)-MS2-hA3a D131E

    Plasmid

    #112132

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131E

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131E mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D131E mutant in hA3a

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSFDuet-SUMO-h-cGAS(T321I)

    Plasmid

    #232294

    Purpose

    Expression of mutant human cGAS protein (T321I)

    Depositor

    Thomas Tuschl

    Article

    Skeldon et al Commun Chem. 2025 Mar 22;8(1):88. doi: 1

    Insert

    MB21D1 (CGAS )
    

    Mutation

    T321I

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge