We narrowed to 9,425 results for: try

1. pDONR207 SARS-CoV-2 E 27nt-del_nostop

   Plasmid

   #153955

   Purpose

   Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   E (E SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   June 23, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDONR223 SARS-CoV2 TEV-NSP8

   Plasmid

   #154407

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

   Plasmid

   #105814

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MK08_HUMAN_D0

   Plasmid

   #79695

   Purpose

   This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK08 (MAPK8 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCDF-casBCDE(+6)

   Plasmid

   #89728

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a longer versi…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-ITGB1(no stop)

   Plasmid

   #215453

   Purpose

   Gateway entry vector with integrin beta1 wild-type (WT) without a stop codon

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Silent mutations added to disrupt shRNA binding a…

   Promoter

   none

   Available since

   Sept. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pENTR2b-PTPN11(D425A,C459S)-Clover

   Plasmid

   #215520

   Purpose

   Gateway entry vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   PTPN11 (PTPN11 Human)
   

   Use

   Gateway entry vector

   Tags

   Clover

   Expression

   Mutation

   D425A, C459S (Phosphatase inactive)

   Promoter

   none

   Available since

   Sept. 3, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.1(+)_Nav1.6(K1425TAG)-HA

   Plasmid

   #239763

   Purpose

   Expresses mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Scn8a (Scn8a Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   K1425TAG

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)_Nav1.6(K1546TAG)-HA

   Plasmid

   #239764

   Purpose

   Expresses mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Scn8a (Scn8a Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   K1546TAG

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)_Nav1.6(WT, Y371C)-HA

    Plasmid

    #239765

    Purpose

    Expresses TTX-resistant (Y371C) variant of mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Y371C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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