We narrowed to 16,131 results for: form

1. Sema6a.a-Fc-His

   Plasmid

   #72163

   Purpose

   Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6a.a (Sema6a Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR221-TAZ CA

   Plasmid

   #79504

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   3xFLAG

   Mutation

   S89A

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-neo-mApg5(K130R)

   Plasmid

   #22957

   Depositor

   Noboru Mizushima

   Article

   Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

   Insert

   autophagy related genes 5 (Atg5 Mouse)
   

   Expression

   Mammalian

   Mutation

   130 Lysine to Arginine

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

   Plasmid

   #108737

   Purpose

   Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa270
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

   Plasmid

   #123188

   Purpose

   binary plant vector for transient fLUC (with intron) expression

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
   

   Use

   Luciferase

   Expression

   Plant

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-neo-(HA)3-ε

   Plasmid

   #198531

   Purpose

   Expression of HA-tagged AP-4 ε in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

   Insert

   AP-4 ε (AP4E1 Human)
   

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   silent substitutions in codons 235, 262, 905 and …

   Promoter

   CMV

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dscam1_7.27.30 EC10-Fc-His

   Plasmid

   #72190

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.30
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-CH115gp160-QES.c14-754*

   Plasmid

   #123282

   Purpose

   Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (CH115) Env
   

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Premature stop co…

   Promoter

   CMV

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-FLAG-NEMO-C54/347A

   Plasmid

   #27268

   Depositor

   Thomas Gilmore

   Article

   Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

   Insert

   NEMO (IKBKG Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Cys54Ala Cys347Ala

   Available Since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

10. Ncam1_1.0.1-AP-His

    Plasmid

    #71961

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_1.0.1 (Ncam1 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 GABARAPL2 G116

    Plasmid

    #123088

    Purpose

    Gateway entry clone encoding human GABARAPL2 G116

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. actc1b-mKate2-EEA1

    Plasmid

    #109508

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    EEA1 (eea1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3/flag-pBirA(1-G78)-FRB

    Plasmid

    #154447

    Purpose

    expresses pBirA(1-G78)-FRB/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pBirA(1-G78)-FRB
    

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

    Plasmid

    #86195

    Purpose

    U6 based expression of N meningitidis sgRNA

    Depositor

    Scot Wolfe

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    nmCas9 sgRNA cloning cassete
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA

    Plasmid

    #108773

    Purpose

    Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa269
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

    Plasmid

    #108774

    Purpose

    Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa270 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCHKO_HPRT1_exon3_2_Lb

    Plasmid

    #155054

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQC hKGA.wt V5-His IRES G418

    Plasmid

    #110390

    Purpose

    γ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    KGA glutaminase (GLS Human)
    

    Use

    Retroviral

    Tags

    6xHis and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRX-IP/SECFP-mATG5

    Plasmid

    #58744

    Purpose

    Expresses /SECFP-ATG5 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Retroviral

    Tags

    SECFP

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

20. IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA

    Plasmid

    #121799

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + CMV::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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