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pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199450

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

Plasmid

#199451

Purpose

CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

Plasmid

#199452

Purpose

CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_B1ChR2_TS_mScarlet_ER

Plasmid

#195191

Purpose

Highly K+ selective channelrhodopsin

Depositor

Peter Hegemann, Johannes Vierock

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Insert

Bilabrum sp. K+ selective channelrhodopsin 2

Tags

ER-export sequence, Kir2.1 trafficking motif, and…

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-M.Fas.1

Plasmid

#196687

Purpose

Rep/Cap plasmid for the production of M.Fas.1, an AAV capsid with CNS tropism in macaques.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

TDALTTK insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-M.Fas.4

Plasmid

#196690

Purpose

Rep/Cap plasmid for the production of M.Fas.4, an AAV capsid with CNS tropism in macaques.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

VVSDYTV insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-M.Fas.3

Plasmid

#196689

Purpose

Rep/Cap plasmid for the production of M.Fas.3, an AAV capsid with CNS tropism in macaques.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RVDPSGL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-mini v2-HF24_F4

Plasmid

#195718

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#4 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F20

Plasmid

#195734

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#20 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F5

Plasmid

#195719

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#5 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-83-GB1_AD

Plasmid

#148513

Purpose

Bacterial Expression of DmeIF4Ga_617-630-DmThor_64-83

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4Ga_617-630-DmThor_64-83 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso2-E2302AI2313R_U

Plasmid

#147734

Purpose

Mammalian Expression of HsNot1iso2-E2302AI2313R

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso2-E2302AI2313R (CNOT1 Human)

Expression

Mammalian

Mutation

4 silent and two non silent G917E and R2353Q muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso2-R2097DI2313R_U

Plasmid

#147735

Purpose

Mammalian Expression of HsNot1iso2-R2097DI2313R

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso2-R2097DI2313R (CNOT1 Human)

Expression

Mammalian

Mutation

4 silent and two non silent G917E and R2353Q muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3-del698-725-dsRNAres_U

Plasmid

#147718

Purpose

Insect Expression of DmNot3-del698-725-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot3-del698-725-dsRNAres (Not3 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3-del698-725-F764E-dsRNAres_U

Plasmid

#147719

Purpose

Insect Expression of DmNot3-del698-725-F764E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot3-del698-725-F764E-dsRNAres (Not3 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3-del723-745-dsRNAres_U

Plasmid

#147720

Purpose

Insect Expression of DmNot3-del723-745-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot3-del723-745-dsRNAres (Not3 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsNot3_666-753_U

Plasmid

#147722

Purpose

Bacterial Expression of HsNot3_666-753

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_666-753 (CNOT3 Human)

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso2-3xmut_U

Plasmid

#147731

Purpose

Mammalian Expression of HsNot1iso2-3xmut

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso2-3xmut (CNOT1 Human)

Expression

Mammalian

Mutation

4 silent and two non silent G917E and R2353Q muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso2-8xmut_U

Plasmid

#147733

Purpose

Mammalian Expression of HsNot1iso2-8xmut

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso2-8xmut (CNOT1 Human)

Expression

Mammalian

Mutation

4 silent and two non silent G917E and R2353Q muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot3-del698-725-F764E-dsRNAres_U

Plasmid

#147704

Purpose

Insect Expression of DmNot3-del698-725-F764E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot3-del698-725-F764E-dsRNAres (Not3 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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