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[#GS16-LV] Syn1-MitoTRACER - Donor

Plasmid

#233565

Purpose

Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Lentiviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-NE2h-IRES-mCherry-CAAX

Plasmid

#208691

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2h

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

Plasmid

#149276

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

Plasmid

#223712

Purpose

Expresses autophagy flux LC3B fluorescence reporter

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

LC3B (MAP1LC3B Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

mCherry::EGFP::LC3B

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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PGC-1 alpha promoter 2kb luciferase

Plasmid

#8887

Depositor

Bruce Spiegelman

Article

Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

Insert

PGC-1 alpha promoter (Ppargc1a Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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EGFP-E-Syt1

Plasmid

#66830

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD

Plasmid

#235576

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylation

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Dnmt3a-Dnmt3L (Dnmt3a, Dnmt3l Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-NE2m-IRES-mCherry-CAAX

Plasmid

#208690

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

Plasmid

#162280

Purpose

Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN8A Variant 1, stabilized (SCN8A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

Modified human HSPA5 intron 4 inserted after bp23…

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-BcPC-PLC

Plasmid

#224255

Purpose

Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

Depositor

Chiaki Murakami

Article

Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

Insert

Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)

Tags

GST and HRV-3C (LEVLFQGP) site

Expression

Bacterial

Mutation

The gene of Bacillus cereus ATCC 14579 was used f…

Promoter

lac promoter

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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wtBxb1

Plasmid

#222337

Purpose

Plasmid for expressing wild type Bxb1 in mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized wild type Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - HA-KLF4 FL

Plasmid

#34593

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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CS2+MYC-Etv5:T139D

Plasmid

#49097

Purpose

expression of Etv5 T139D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 T139D (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Tags

MYC

Expression

Mammalian

Mutation

T139D

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

Plasmid

#162279

Purpose

Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN2A Variant 1, stabilized (SCN2A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

IVS intron inserted after bp4551.

Promoter

CMV

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p(HA)HIF1alpha (P402A,P564A)

Plasmid

#52636

Purpose

mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

Depositor

Eric Huang

Article

Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

Insert

HIF1alpha P402A, P564A (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

P402A, P564A

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb

Plasmid

#238232

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-p65

Plasmid

#111190

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

RELA (RELA Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta3-3xFiBsd

Plasmid

#140962

Purpose

full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis associated 3 (Mta3 Synthetic, Mouse)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-E-Syt2

Plasmid

#66831

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt2 (ESYT2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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