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pAV0749

Plasmid

#133483

Purpose

Expression in fission yeast from promoter ptdh1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt1

Plasmid

#213628

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 (Syt1 Rat)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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WFS1 P724L-Flag-pcDNA3

Plasmid

#13012

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed P 724 to L

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only
Twist-siRNA5

Plasmid

#8456

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siTwist (Twist1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pSB700 HL1gR4

Plasmid

#124450

Purpose

Expresses gRNA HL1 gR4 in the pSB700 backbone

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

HL1 gR4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKMV-VioC

Plasmid

#65349

Purpose

systhesis of vioC gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MTUS1 zeo

Plasmid

#192323

Purpose

Lentiviral expression vector for an inducible 3xFLAG-MTUS1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

MTUS1 (MTUS1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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E12 2/5-pCS2

Plasmid

#20913

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 2/5 (TCF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pYFAC

Plasmid

#168982

Purpose

Filamentous fungi vector. Also referred to as pYFAC-pyrG.

Depositor

Yit Heng Chooi

Article

Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFUSE-rabbitFc1-IL2ss-VHH(B12)-His-Myc

Plasmid

#209837

Purpose

Plasmid coding for a nanobody against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

Depositor

Tim Brown

Inserts

IL2-ss
VHH (B12 variant)
Rabbit Fc Gamma 1

Use

Tags

x3 MycTag and x6 HisTag

Expression

Mammalian

Mutation

Promoter

hEF1-HTLV promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Geph CR3m shRNA

Plasmid

#121069

Purpose

Negative control shRNA for plasmid 121068: shRNA targeting the coding region of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Dendra2-Gamma-Tubulin-17

Plasmid

#57721

Purpose

Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Gamma-Tubulin (TUBG1 Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMOD_A1511

Plasmid

#91037

Purpose

Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

OsAct1

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only
P303-FBP_2

Plasmid

#139693

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAG260

Plasmid

#33210

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

SYNM (SYNM Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
gRNA-mCtnnb1

Plasmid

#84542

Purpose

mCtnnb1 gRNA expressing vector

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mCtnnb1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf9

Plasmid

#163599

Purpose

Expresses GDF-9 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf9 (Gdf9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B BimEL (3SA)

Plasmid

#23091

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (3SA) (Bcl2l11 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 55/65/73 to alanine

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
c-myc promoter (4x TBE2 - wt)

Plasmid

#16599

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE2 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only