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pCMV-HA-mIFITM3

Plasmid

#58389

Purpose

Expressed murine IFITM3 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
3xnls-mTurquoise2

Plasmid

#98817

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEx-deletion-erm

Plasmid

#172213

Purpose

For allelic exchange in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pKH118_keima-YIPF4

Plasmid

#214971

Purpose

Golgiphagy flux reporter keima-YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

keima-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-cortactin

Plasmid

#26722

Depositor

Anna Huttenlocher

Article

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-mStayGold

Plasmid

#229802

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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mc2 neo

Plasmid

#206214

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLPCX-NICD

Plasmid

#44471

Depositor

Ernesto Canalis

Article

Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

Insert

Notch intracellular domain (Notch1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Contains amino acid 1753 to C-Term

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only
Bxb1-GT_CAG-mRuby2

Plasmid

#229801

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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ITPKA-mNeonGreen

Plasmid

#98883

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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TL024_lenti_hSyn:HaloTag-GluA1

Plasmid

#192517

Purpose

Expression of AMPA GluA1 with N terminal HaloTag fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

HaloTag-GluA1

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

hSyn

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM60-Ptb

Plasmid

#38245

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Ptb (Ptbp1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F2

Plasmid

#184167

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F1

Plasmid

#184166

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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PEP108E

Plasmid

#97394

Purpose

binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Plasma membrane target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTfR-mNeonGreen

Plasmid

#129608

Purpose

In vivo visualization of Transferrin receptors (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Transferrin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBlu/gRNA

Plasmid

#59188

Purpose

Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Guide RNA Cassette

Use

CRISPR

Expression

Plant

Promoter

U6 (Arabidopis)

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCIP-caTfeb

Plasmid

#79013

Purpose

For mammalian expression of a shortened, constitutively active version of Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Tfeb (TFEB Human)

Expression

Mammalian

Mutation

Removed first ~400 bp from 5' end (N-terminu…

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only
pIVT-Bxb1

Plasmid

#232746

Purpose

IVT template for making modRNA encoding Bxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKIBra-P2A-mCherry-Puro-sfGFP

Plasmid

#115686

Purpose

Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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