We narrowed to 16,583 results for: EMB

1. AviTag-SpyTag003-MBP

   Plasmid

   #184227

   Purpose

   Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   AviTag-SpyTag003-MBP
   

   Use

   Tags

   AviTag, His6, SpyTag003, and Thrombin cleavage si…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cav1-mRFP

   Plasmid

   #14434

   Depositor

   Ari Helenius

   Article

   Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

   Insert

   Caveolin-1 (CAV1 Canis familalis)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME EFS-rtTA/rtTA3 (JDW 1230)

   Plasmid

   #224525

   Purpose

   A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   EFS-rtTA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

   Plasmid

   #86419

   Purpose

   C-terminal PCR tagging module for yeast codon optimized split GFP1-10

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   GFP1-10
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GB-Dcp1b

   Plasmid

   #153979

   Purpose

   Dimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1b

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1b (DCP1B Human)
   

   Use

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-mitoT

   Plasmid

   #174519

   Purpose

   Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

   Depositor

   Oleh Khalimonchuk

   Article

   Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

   Insert

   MitoT synthetic tether
   

   Use

   Tags

   6xHis and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-hs_ICAM_short (JDW 1046)

   Plasmid

   #224480

   Purpose

   Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   min-ICAM2 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPI/hPLK2WT/Neo

   Plasmid

   #35385

   Depositor

   Anurag Tandon

   Article

   Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

   Insert

   hPLK2 (PLK2 Human)
   

   Use

   Lentiviral

   Tags

   EMCV IRES-Neomycin cassette and V5 epitope and Po…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ICUE3

   Plasmid

   #61622

   Purpose

   FRET-based biosensor for monitoring cAMP

   Depositor

   Jin Zhang

   Article

   DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

   Insert

   ICUE3 (RAPGEF3 Human)
   

   Use

   Tags

   ECFP and cpVenus[L194]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. ECFP-GalT

    Plasmid

    #11937

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINB-OTUB1*

    Plasmid

    #65441

    Purpose

    Expresses activated OTUB1 in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    UbcH5b (UBE2D2 Human)
    otubain-1 (OTUB1 )
    

    Use

    Tags

    His-3C

    Expression

    Bacterial

    Mutation

    Active site cysteine mutated to alanine

    Promoter

    T7

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. qTAG-AAVS1-Ef1a-Zeo

    Plasmid

    #227268

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA_RGG-GFP-RGG

    Plasmid

    #124930

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-3xFLAG-Rbfox3

    Plasmid

    #213727

    Purpose

    Mammalian cell expression vector for overexpression of Rbfox3 in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    Rbfox3 (Rbfox3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. YFP-PML-IV-mSIM-C3

    Plasmid

    #134529

    Purpose

    Expresses Cherry-PML-IV fusion protein with a mutation in its SIM

    Depositor

    Susan Janicki

    Article

    Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

    Insert

    PML-IV-mSIM
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    SIM (VVVI) 556-559 aa changed to VGGG

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. doraWT_rescue_construct

    Plasmid

    #190609

    Purpose

    Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. μ2-HA-D176A

    Plasmid

    #32754

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D176A

    Promoter

    CMV

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pET_RGG-RGG-RGG

    Plasmid

    #124938

    Purpose

    Expression of RGG-RGG-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Cas13d-T2A-GFP(1-10)

    Plasmid

    #224566

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-GFP(1-10)
    

    Use

    CRISPR

    Tags

    T2A-split GFP(1-10)

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-nucGFP11-3xControlgRNA

    Plasmid

    #224568

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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