We narrowed to 15,735 results for: LEA

1. MZB133

   Plasmid

   #228484

   Purpose

   "Empty" N-terminal TEV cleavable 6x His tag vector for rapid cloning/DNA Assembly via SmaI restriction site (Bacterial Expression)

   Depositor

   Richard Baker

   Type

   Empty backbone

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGGsH6_MCS_SHORT_outFrame

   Plasmid

   #211439

   Purpose

   Vector backbone for random polymerase chain reaction screen

   Depositor

   Helmut Pospiech

   Insert

   MCS-tev cleavage site-EGFP-6xHis-Tag
   

   Expression

   Bacterial

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. U4m

   Plasmid

   #121017

   Purpose

   MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   UTR1
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. C77m

   Plasmid

   #120989

   Purpose

   MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   AmpR
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. C78m

   Plasmid

   #120990

   Purpose

   MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ChlorR
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His10 TEV LIC cloning vector (2B-T-10)

   Plasmid

   #78173

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   10xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_SFFV_H2B_miRFP703

   Plasmid

   #231951

   Purpose

   Lentiviral vector for constitutive expression of the far-red fluorescent protein miRFP703 fused with the H2B nuclear localization tag (SFFV promoter)

   Depositor

   Leonardo Morsut

   Insert

   H2B_miRFP703
   

   Use

   Lentiviral

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOpen-Eco31I

   Plasmid

   #165506

   Purpose

   Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   Eco31I
   

   Use

   Synthetic Biology

   Available Since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pOpen-9N7polA

   Plasmid

   #165500

   Purpose

   Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   9 degrees N-7 DNA polymerase
   

   Use

   Synthetic Biology

   Available Since

   July 26, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. MZB117

    Plasmid

    #228481

    Purpose

    "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Tags

    GST (glutathione S-transferase)

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOpen-UDG

    Plasmid

    #165531

    Purpose

    Uracil DNA glycosylase catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    UDG
    

    Use

    Synthetic Biology

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET28GST-LIC

    Plasmid

    #26101

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6XHis, GST, and Thrombin CLeavage Site

    Expression

    Bacterial

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. MZB206

    Plasmid

    #228493

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable MSP2N2 for biotinylated nanodiscs

    Depositor

    Richard Baker

    Insert

    MSP2N2
    

    Tags

    10xHis-SUMO(Smt3)-Avitag-HRV

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSV40_HA-mPACT

    Plasmid

    #120537

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    PACT (Prkra Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOpen-T4 PGD

    Plasmid

    #165550

    Purpose

    T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    T4 PGD (T4 Endonuclease V)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. mApple-FTR-940

    Plasmid

    #54902

    Purpose

    Localization: Nuclear - Nucleolus, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    FTR
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. P18m

    Plasmid

    #120941

    Purpose

    MoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    T7
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. C86m

    Plasmid

    #120997

    Purpose

    MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    X174E
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. P9m

    Plasmid

    #120937

    Purpose

    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file

    Depositor

    Richard Murray

    Insert

    Pr-OR1-OR2
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tdTomato-FTR-940

    Plasmid

    #58094

    Purpose

    Localization: Nuclear - Nucleolus, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    FTR
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only