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p131C-B10

Plasmid

#241908

Purpose

Optimised PCA biosensor produced sfGFP in response to hydroxyl-substituted benzoic acids, including protocatechuic acid (PCA)

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

pcaV, sfGFP

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEEK1

Plasmid

#83488

Purpose

Expression plasmid for conjugational transfer into cyanobacteria

Depositor

Pia Lindberg

Article

Kukil et al Microb Cell Fact. 2022 Jan 10;21(1):8. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Strep-tag

Expression

Bacterial

Promoter

Ptrc2O

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT24

Plasmid

#200998

Purpose

pLv2-pUC18R6KT-miniTn7-aad9, SpR, StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK1056

Plasmid

#209546

Purpose

BTK Type 4 antibiotic resistance cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Type 4 Kanamycin resistance cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAM750

Plasmid

#207889

Purpose

Level 0 for A3 module (5' TCCC-3' AACA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM749

Plasmid

#207888

Purpose

Level 0 for AD2 module (5' TGAC-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM758

Plasmid

#207897

Purpose

Level 0 for C4 module (5' GCAG-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM756

Plasmid

#207895

Purpose

Level 0 for C2 module (5' AGCC-3' TTCG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM757

Plasmid

#207896

Purpose

Level 0 for C3 module (5' TTCG-3' GCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM745

Plasmid

#207887

Purpose

Level 0 for A1 module (5' ACCT-3' TGAC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM755

Plasmid

#207894

Purpose

Level 0 for C1 module (5' GGCT-3' AGCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0013

Plasmid

#205582

Purpose

NpuDnaE(C)_HYG(53-341)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Let7-SunTag

Plasmid

#196928

Purpose

Let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-TREAT

Plasmid

#196921

Purpose

The 5UTR of the ferritin heavy chain mRNA was cloned upstream of the Renilla coding sequence in the ΔIRE-TREAT reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Let7R-SunTag

Plasmid

#196929

Purpose

Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-B-frSkyran-S

Plasmid

#182209

Purpose

Bacterial expression of frSkylan-S

Depositor

Shiro Suetsugu

Article

Osuga et al Mol Biol Cell. 2021 Nov 1;32(21):br7. do

Insert

frSkylan-S

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJC20_cam1-T6C

Plasmid

#182427

Purpose

Bacterial expression of the Schizosaccharomyces pombe fission yeast calmodulin, Cam1 with the T6C residue replacement to allow labelling

Depositor

Dan Mulvihill

Article

Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

Insert

cam1 (cam1 Fission Yeast)

Expression

Bacterial

Mutation

T6C substitution

Promoter

T7

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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OA959C

Plasmid

#104968

Purpose

Expresses anti-ZIKV transgene in Ae. aegypti

Depositor

Omar Akbari

Article

Buchman et al Proc Natl Acad Sci U S A. 2019 Feb 5. pi

Insert

Anti-Zika Transgene

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1

Plasmid

#113943

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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