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AA428

Plasmid

#216055

Purpose

Fragmid fragment: (C' terminus) NpNLS; 3xHA; c-mycNLS; c-mycNLS

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

NpNLS_v1.1; 3xHA_v1.1; c-mycNLS_v1.2; c-mycNLS_v1.3

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA106

Plasmid

#209339

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.

Depositor

Lucia Strader

Publication

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pML646

Plasmid

#206999

Purpose

Contains mAID-tag, mouse Ptbp1-specific homology arms and blasticidin S resistance gene.

Depositor

Eugene Makeyev

Publication

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

BSD resistance

Use

Unspecified

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_EF-NR

Plasmid

#202179

Purpose

Level 0 plasmid containing a terminator sequence from gene 54983 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-HO

Plasmid

#202170

Purpose

Level 0 plasmid containing a terminator sequence from gene 5902 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

HO terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AF-spacer

Plasmid

#202130

Purpose

Level 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Spacer

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-NR

Plasmid

#202123

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-HO

Plasmid

#202116

Purpose

Level 0 plasmid containing the promoter from gene 5902 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

HO promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-NR

Plasmid

#202101

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-H4

Plasmid

#202100

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1_SV40-iBlue-pA

Plasmid

#200234

Purpose

Backbone for cloning iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB302-er-mCherry

Plasmid

#186287

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Publication

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Expression

Bacterial

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_37_pKD46.10

Plasmid

#178927

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

March 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_26.10

Plasmid

#178919

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

March 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_15a.10

Plasmid

#178913

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

Feb. 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pYI047

Plasmid

#170843

Purpose

(pFRO6::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Publication

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pFRO6::SMT2-FLAG

Expression

Plant

Promoter

FRO6p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI048

Plasmid

#170844

Purpose

(pGH3.17::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Publication

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pGH3.17::SMT2-FLAG

Expression

Plant

Promoter

GH3.17p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pOD_004

Plasmid

#155361

Purpose

Gentamycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

Depositor

Elisabeth Raleigh

Publication

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_67.10

Plasmid

#178940

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_47_pKD46.10

Plasmid

#178932

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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