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pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

Plasmid

#199355

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-SUMOstar

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

Plasmid

#199365

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS

Plasmid

#199352

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-3C

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

Plasmid

#199364

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

Plasmid

#199368

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOstar-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS117 His14-Avi-45xGS-anti GFP nanobody

Plasmid

#199370

Purpose

Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-GFP nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

Plasmid

#199371

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 BS (-) N protein

Plasmid

#188448

Purpose

SARS CoV-2 N protein expression

Depositor

Ajit Chande

Article

Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

Insert

SARS-CoV-2 N protein

Tags

Strep-Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Promoter

T5-LacO

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHub10-His-YFP-PIP2;1

Plasmid

#99386

Purpose

overexpression and isolation of recombinant At-PIP2;1 protein

Depositor

Ning Li

Article

Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

Insert

PIP2;1 (PIP2A Mustard Weed)

Tags

His and YFP

Expression

Plant

Promoter

2xCaMV35S

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pADSL-2XStrep

Plasmid

#125684

Purpose

expresses human ADSL protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

ADSL (ADSL Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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FMR1-E17B-P2A-NLuc

Plasmid

#157857

Purpose

donor plasmid for FMR1-Nluc

Depositor

Xinyu Zhao

Article

Li et al Stem Cells. 2017 Jan;35(1):158-169. doi:

Insert

FMR1-Nlu (FMR1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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UASp-deltaN cycB

Plasmid

#112967

Purpose

P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Lee et al Genetics. 2001 Aug;158(4):1545-56.

Insert

cycB (CycB Fly)

Use

P insertion vector

Mutation

N-term deletion of first 46 amino acids, includes…

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-p15 Bid-KKAA-GFP

Plasmid

#191970

Purpose

Expression of the active p15 cBid KKAA mutant, chimera with GFP.

Depositor

Luca Scorrano

Insert

p15 - cBid KKAA

Expression

Mammalian

Mutation

KKAA: Lys 157 and Lys 158 into Ala

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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ADSL_pLX307

Plasmid

#98311

Purpose

Lentiviral expression of ADSL

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ADSL (ADSL Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pUC-MLXγ

Plasmid

#211755

Purpose

Cloning vector for the expression of mouse MLXγ

Depositor

Russell Goodman

Article

Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

Insert

MLX (Mlx Mouse)

Use

Cloning vector

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-ccdB-6stop

Plasmid

#203511

Purpose

Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416GAL10-ccdB-6stop

Plasmid

#203512

Purpose

Gateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416GALL-HIV-1_PR

Plasmid

#203493

Purpose

Expresses HIV-1 protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HIV-1 protease

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->AA

Plasmid

#226600

Purpose

4 glutamine pairs to alanine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->AA (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with AA pairs at positions 27…

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->GG

Plasmid

#226601

Purpose

4 glutamine pairs to glycine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->GG (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with GG pairs at positions 27…

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->NN

Plasmid

#226602

Purpose

4 glutamine pairs to asparagine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->NN (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 glutamine pairs to asparagine pairs at position…

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-LV_3CL

Plasmid

#203507

Purpose

Expresses LV 3CL protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

LV 3CL protease

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HTLV_PR

Plasmid

#203467

Purpose

Expresses HTLV protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HTLV protease

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-EYFP

Plasmid

#201792

Purpose

Expresses EYFP from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

EYFP

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUC-ChREBPα

Plasmid

#211754

Purpose

Cloning vector for the expression of mouse ChREBPα

Depositor

Russell Goodman

Article

Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

Insert

MLXIPL (Mlxipl Mouse)

Use

Cloning vector

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HRV-B14_3C

Plasmid

#203466

Purpose

Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HRV-B14 3C protease (HRVBgp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-EMCV_3C

Plasmid

#201941

Purpose

Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

EMCV 3C protease (EMCVgp1 )

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416GAL-3x-uC-ccdB-6stop

Plasmid

#203514

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416GAL-1x-ccdB-6stop

Plasmid

#203519

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge