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5-HT2C_MSV

Plasmid

#252887

Purpose

Mammalian expression of RNA edited version of human 5-HT2C

Depositor

Cesare Orlandi

Article

Bonniwell et al ACS Chem Neurosci. 2025 Oct 1;16(19):389

Insert

5-HT2C (HTR2C Human)

Tags

HA tag, inserted after signal peptide

Expression

Mammalian

Mutation

MSV isoform

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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5-HT2C_IGI

Plasmid

#252882

Purpose

Mammalian expression of RNA edited version of human 5-HT2C

Depositor

Cesare Orlandi

Article

Bonniwell et al ACS Chem Neurosci. 2025 Oct 1;16(19):389

Insert

5-HT2C (HTR2C Human)

Tags

HA tag, inserted after signal peptide

Expression

Mammalian

Mutation

IGI isoform

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pMDC32B-BS-AtMIR390a-A18G-B/c

Plasmid

#246716

Purpose

Cloning amiRNA sequences in AtMIR390a-A18G precursors for in planta expression

Depositor

Alberto Carbonell

Article

Llorens-Gamez et al Plant J. 2026 Jan;125(1):e70665. doi: 10

Insert

Basal stem of Arabidopsis MIR390a precursor with A18G mutation & chloramphenicol-ccdB cassette flanked by two BsaI sites (MIR390a Mustard Weed)

Use

RNAi

Expression

Plant

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Eco1

Plasmid

#240705

Purpose

pLAM12 vector backbone containing Eco1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al Nat Biotechnol (2026). https://doi.org/1

Insert

Eco1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Eco2

Plasmid

#240706

Purpose

pLAM12 vector backbone containing Eco2 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al Nat Biotechnol (2026). https://doi.org/1

Insert

Eco2 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Tsp1

Plasmid

#240712

Purpose

pLAM12 vector backbone containing Tsp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al Nat Biotechnol (2026). https://doi.org/1

Insert

Tsp11 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Kva1

Plasmid

#240709

Purpose

pLAM12 vector backbone containing Kva1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al Nat Biotechnol (2026). https://doi.org/1

Insert

Kva1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Hcl1

Plasmid

#240714

Purpose

pLAM12 vector backbone containing Hcl1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al Nat Biotechnol (2026). https://doi.org/1

Insert

Hcl1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Eas1

Plasmid

#240710

Purpose

pLAM12 vector backbone containing Eas1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al Nat Biotechnol (2026). https://doi.org/1

Insert

Eas1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Csp1

Plasmid

#240711

Purpose

pLAM12 vector backbone containing Csp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al Nat Biotechnol (2026). https://doi.org/1

Insert

Csp1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Abe1

Plasmid

#240708

Purpose

pLAM12 vector backbone containing Abe1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al Nat Biotechnol (2026). https://doi.org/1

Insert

Abe1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Ban1

Plasmid

#240707

Purpose

pLAM12 vector backbone containing Ban1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al Nat Biotechnol (2026). https://doi.org/1

Insert

Ban1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CCR5

Plasmid

#222309

Purpose

Synchronize the trafficking of CCR5 from the ER.

Depositor

Franck Perez

Article

Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

Insert

Streptavidin-KDEL and CCR5 fused to SBP-EGFP (CCR5 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

Plasmid

#195054

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pLDP1

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

Plasmid

#195053

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pFBA

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

Plasmid

#195052

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pXYL

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

Plasmid

#195051

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pGPD

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

Plasmid

#195050

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pADH2

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-ε ear

Plasmid

#196670

Purpose

Expresses GST-AP-4 ε ear (residues 840-1137) in bacteria

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε ear (840-1137) (AP4E1 Human)

Tags

GST

Expression

Bacterial

Mutation

silent mutations in codons 905, 1129 and 1137

Promoter

tac

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-TSF-ε

Plasmid

#196583

Purpose

Expresses the two Strep/one FLAG (TSF)-tagged human AP-4 ε subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε subunit (AP4E1 Human)

Tags

two Strep/one FLAG (TSF)

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-γ1

Plasmid

#197078

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-1 γ1 (Ap1g1 Mouse)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-1

Plasmid

#180394

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-1 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-2

Plasmid

#180395

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-2 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV.CBA.YFP.miR-E_shTgm5-3

Plasmid

#180396

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-3 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
shFGF2#1

Plasmid

#157667

Purpose

Tet inducible knockdown of FGF2

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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shAPI5#2

Plasmid

#157666

Purpose

Tet inducible knockdown of API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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shAPI5#1

Plasmid

#157665

Purpose

Tet inducible knockdown of API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-API5

Plasmid

#157660

Purpose

Expresses API5 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

GST tag

Expression

Bacterial

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
INCTbiosyn-pUB-HspINT4

Plasmid

#127513

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 4.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 4 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT5

Plasmid

#127514

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 5.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 5 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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