Addgene

We narrowed to 8,145 results for: FIE

Showing: 821 - 840 of 8145 results

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pHAT2

Plasmid

#112583

Purpose

Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

Depositor

Johan Peränen, Marko Hyvönen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pwM

Plasmid

#22515

Depositor

Christopher Buck

Article

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Insert

MCPyV VP1

Use

Tags

Expression

Mammalian

Mutation

codon modified gene

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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psi-CHECK3

Plasmid

#136010

Purpose

psi-CHECK2 plasmid modified so both Renilla and Firefly luciferase contain introns

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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ph2m

Plasmid

#22518

Depositor

Christopher Buck

Article

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Insert

MCPyV VP2

Use

Tags

Expression

Mammalian

Mutation

codon modified

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pET21TtTERT(195-516)

Plasmid

#28172

Depositor

Kathleen Collins

Article

O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

Insert

Tetrahymena TERT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pAc5.1C-FLuc-Stop-5BoxB

Plasmid

#21301

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

5BoxB

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMAK462

Plasmid

#140700

Purpose

NBαMs/Rt-IgG1-Fc

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαMs/Rt-IgG1-Fc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGAT3

Plasmid

#112589

Purpose

Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-GST fusions

Depositor

Johan Peränen, Marko Hyvönen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Use

Tags

6His-GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGAT2

Plasmid

#112588

Purpose

Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-GST fusions

Depositor

Johan Peränen, Marko Hyvönen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Use

Tags

6His-GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHAT4

Plasmid

#112585

Purpose

Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

Depositor

Johan Peränen, Marko Hyvönen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMAK463

Plasmid

#140701

Purpose

NBαMs-IgK

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαMs-IgK

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBLO43.3.-6_human ProCas9Flavi-6

Plasmid

#121630

Purpose

U6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCETF

Plasmid

#19783

Depositor

Jumi Shin

Article

Chen et al Biotechniques. 2008 Sep . 45(3):295-304.

Type

Empty backbone

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pCasY1

Plasmid

#87687

Purpose

p15A plasmid expressing CasY1

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasY1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCETT

Plasmid

#19782

Depositor

Jumi Shin

Article

Chen et al Biotechniques. 2008 Sep . 45(3):295-304.

Type

Empty backbone

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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crestin

Plasmid

#89394

Purpose

For in situ hybridization, linearize with Sac I use T7 polymerase

Depositor

Marnie Halpern

Article

Rubinstein et al Genesis. 2000 Jan;26(1):86-97.

Insert

crestin (crestin Zebrafish)

Use

In situ probe

Tags

Expression

Mutation

Promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAY02

Plasmid

#13043

Depositor

Peggy Hsieh

Article

Yamamoto et al Nucleic Acids Res. 2000 Sep 15. 28(18):3

Insert

MutS

Use

Tags

His6

Expression

Bacterial

Mutation

T193TT to G193CT Phe to Ala mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pBLO43.3_human ProCas9Flavi

Plasmid

#121629

Purpose

U6-sgRNAdest_CMV-intron_hProCas9Flavi_T2A Mcherry_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pPS1307

Plasmid

#8912

Depositor

Pamela Silver

Article

Henry et al Mol Cell Biol. 1996 Jul . 16(7):3668-78.

Insert

HMT1 (HMT1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only

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12X1C mAGNET

Plasmid

#67956

Purpose

Lentiviral vector coding EGFP followed by 12x miR-128 target sequences

Depositor

Xue Han, Wilson Wong

Article

Sayeg et al ACS Synth Biol. 2015 Apr 13.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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