We narrowed to 18,671 results for: Met;
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pUltra-U6-gRNAs1-5
Plasmid#178569PurposeConstitutive expression of a gRNA-tRNA array for macsGESTALT editingDepositorInsert5 gRNA-tRNA array with U6 promoter
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku80
Plasmid#46958PurposePlasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.DepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
G-CaMP3
Plasmid#22692Purposea single-wavelength GCaMP2-based genetically encoded calcium indicatorDepositorInsertG-CaMP3
ExpressionMammalianMutationN363D mutation (increased fluorescence)Available SinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHBintE
Plasmid#48136PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP26
Plasmid#199769PurposeExpresses split-intein fused MSP26 for circularization during expression (internal His-Tag)DepositorInsertcMSP26
TagsHis6-TagExpressionBacterialMutationinternal His-TagAvailable SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH4-6
Plasmid#199766PurposeExpresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)DepositorInsertcMSPdH46
TagsHis6-TagExpressionBacterialMutationinternal His-TagAvailable SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV: Flag-Akaluc_SV40:PuroR
Plasmid#183048PurposeExpresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.DepositorInsertAkaLuc
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
HLTV-hTRIM21
Plasmid#104973PurposePurification of His-Lipoyl-hTRIM21 protein from bacteriaDepositorInsertTripartite Motif containing 21 (TRIM21 Human)
TagsHis-LipoylExpressionBacterialPromoterT7Available SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ZFN-AAVS1
Plasmid#89707PurposeMammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locusDepositorInsertZFN-AAVS1
ExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLCK-mScarlet3_C1
Plasmid#189771PurposeConstruct for labelling plasma membranes in mammalian cells using LCK-mScarlet3DepositorInsertLCK-mScarlet3
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
ExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-2
Plasmid#129727PurposeExpressing SpCas9 and sgAAVS1-2DepositorInsertSpCas9 and sgAAVS1-2
UseCRISPRTagsT2A-mCherry-P2A-PuroExpressionMammalianPromoterhU6Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup93
Plasmid#87336PurposeTo express human Nup93 in mammalian cellsDepositorInsertNUP93 (NUP93 Human)
TagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHREAC
Plasmid#134908PurposeOverexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantPromoterdouble 35SAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL3-FLAG
Plasmid#15820DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-Giantin_C1
Plasmid#189773PurposeConstruct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusionDepositorInsertmScarlet3-Giantin
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGM3B
Plasmid#110809Purposecontains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genesDepositorInsertAAV3B cap genes, AAV2 rep genes, adenovirus helper genes
UseAAVAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB533_15F11-sfGFP
Plasmid#167526PurposeH4K20me1-mintbody expression vector for mammalian cellsDepositorInsert15F11-scFv
TagssfGFPExpressionMammalianAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only
