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pACYC-Taq

Plasmid

#102793

Purpose

Expresses Taq DNA polymerase under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGL0_65 [StayGold]

Plasmid

#198976

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

StayGold

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDonorGFP(Ptr)

Plasmid

#175578

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

Mini-Tn, GFP cassette

Expression

Bacterial

Mutation

NO

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9-CR4

Plasmid

#62655

Purpose

Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

Cas9 nuclease

Expression

Bacterial

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLH01

Plasmid

#117261

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-Lp_0640-42, sapK-sappR

Use

Other

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCWU6-xylR-E-luciferase

Plasmid

#190721

Purpose

fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

Depositor

Chenggang Wu

Article

Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

Insert

xylR-E-luciferase

Use

Luciferase

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDCAF-ABC

Plasmid

#113652

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB and ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET-29b(+)_NSP4ss_SerM_NucA-myc

Plasmid

#218191

Purpose

Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Article

Rieubon et al protocols.io 2025

Insert

nucA

Tags

NSP4 signal secretion peptide and myc

Expression

Bacterial

Mutation

codon optimized ecoli residues - 22-266

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pGP8A-ccdB

Plasmid

#83883

Purpose

Destination vector for integration at HIS3, expression from the ADH1 promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pNF02-mLychee

Plasmid

#219400

Purpose

Constitutive expression of mLychee in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mLychee

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pNF02-mChartreuse

Plasmid

#219397

Purpose

Constitutive expression of mChartreuse in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mChartreuse

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pNF02-mJuniper

Plasmid

#219398

Purpose

Constitutive expression of mJuniper in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mJuniper

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2pACryGFP

Plasmid

#64022

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

GFP

Use

Gateway

Promoter

alpha-Acrystallin promoter

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pNF02-mLemon

Plasmid

#219399

Purpose

Constitutive expression of mLemon in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mLemon

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGADCg

Plasmid

#20161

Depositor

Peter Uetz

Article

Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

Type

Empty backbone

Tags

Gal4 AD and SV40 NLS

Expression

Yeast

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pON.mCherry

Plasmid

#84821

Purpose

Constitutive Expression plasmid with mCherry fluorescent protein

Depositor

Howard Shuman

Article

Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

Insert

mCherry

Expression

Bacterial

Promoter

Tac Promoter

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDESTtol2pACrymCherry

Plasmid

#64023

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lens

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

mCherry

Use

Gateway

Promoter

alpha-Acrystallin promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLH02

Plasmid

#117262

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-LVISKB_1732-30 , sapK-sappR

Use

Other

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pPTK021-7-PARS

Plasmid

#84990

Purpose

Type 7, Pichia autonomously replicating sequence

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Pichia autonomously replicating sequence

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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