We narrowed to 14,384 results for: nts

1. B-HiFi SpCas9

   Plasmid

   #207362

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207378

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-Blackjack SpCas9-NLS-6xHis

   Plasmid

   #207375

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Blackjack SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 1005-1013 replaced with two glycine

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-BS-AtMIR390a-B/c

   Plasmid

   #199559

   Purpose

   GATEWAY-compatible entry vector

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10

   Insert

   Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
   

   Use

   Gateway-compatible entry vector

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-In18runon-PTPN22

   Plasmid

   #195377

   Purpose

   A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221

   Depositor

   Patrick Concannon

   Article

   Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

   Insert

   PTPN22 (PTPN22 Human)
   

   Use

   Gateway donor vector

   Mutation

   Intron 18-runon PTPN22

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-In18runon-PTPN22

   Plasmid

   #195379

   Purpose

   Mammalian expression of a human PTPN22 mutant (intron 18-runon) with FLAG tag

   Depositor

   Patrick Concannon

   Article

   Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

   Insert

   PTPN22 (PTPN22 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Intron 18-runon PTPN22

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-EGFP-C1-HsNXF-1-R233AR276A_O

   Plasmid

   #147120

   Purpose

   Mammalian Expression of HsNxf-1-R233AR276A

   Depositor

   Elisa Izaurralde

   Article

   Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

   Insert

   HsNxf-1-R233AR276A (NXF1 Human, See Depositor Comments Below)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuetP-HsEDC3_192-228-GST-HsRCK_E

   Plasmid

   #146211

   Purpose

   Bacterial Expression of HsEDC3_192-228-HsRck

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsEDC3_192-228-HsRck (EDC3 Human)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuetP-HsEDC3_192-228-GST-HsRCK-E236Q_E

    Plasmid

    #146212

    Purpose

    Bacterial Expression of HsEDC3_192-228-HsRck-E236Q

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    HsEDC3_192-228-HsRck-E236Q (EDC3 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuetP-HsEDC3_192-228-GST-HsRCK-K135A_E

    Plasmid

    #146213

    Purpose

    Bacterial Expression of HsEDC3_192-228-HsRck-K135A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    HsEDC3_192-228-HsRck-K135A (EDC3 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuetP-HsEDC3_192-508-GST-HsRCK-E236Q_E

    Plasmid

    #146215

    Purpose

    Bacterial Expression of HsEDC3_192-508-HsRck-E236Q

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    HsEDC3_192-508-HsRck-E236Q (EDC3 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuetP-HsEDC3_192-508-GST-HsRCK-K135A_E

    Plasmid

    #146216

    Purpose

    Bacterial Expression of HsEDC3_192-508-HsRck-K135A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    HsEDC3_192-508-HsRck-K135A (EDC3 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-lambdaN-HA-DmMe31B-Q281AH284A_E

    Plasmid

    #146204

    Purpose

    Insect Expression of DmMe31B-Q281AH284A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmMe31B-Q281AH284A (me31B Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation A1101G and two non silent mut…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-lambdaN-HA-DmMe31B-T288AK292A_E

    Plasmid

    #146205

    Purpose

    Insect Expression of DmMe31B-T288AK292A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmMe31B-T288AK292A (me31B Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation A1101G and two non silent mut…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-Ex1-17-PTPN22

    Plasmid

    #195375

    Purpose

    Mammalian expression of a human PTPN22 mutant (exon 1-17) with FLAG tag

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

    Insert

    PTPN22 (PTPN22 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    PTPN22 mutant containing exon 1 to 17

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGADT7-γ1

    Plasmid

    #197078

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-1 γ1 (Ap1g1 Mouse)
    

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV/EF1a::DIO-iLMO2

    Plasmid

    #129482

    Purpose

    AAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Jaiswal et al J Neurosci Res. 2017 Aug 3. doi: 10.1002

    Insert

    iLMO2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

    Plasmid

    #195865

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-intron-DIO-hfCas13d-pA

    Plasmid

    #195866

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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