We narrowed to 18,079 results for: comp

1. pU6-sgRNA-CBh-eGFP

   Plasmid

   #206885

   Purpose

   cloning backbone for sgRNA using OptScf2

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP PICH (Nigg CB62)

   Plasmid

   #41163

   Depositor

   Erich Nigg

   Article

   Baumann et al Cell. 2007 Jan 12;128(1):101-14.

   Insert

   PICH (ERCC6L Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3 IKKE K38A flag

   Plasmid

   #26202

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon K38A (IKBKE Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Catalytic Lysine changed to Alanine (K38A)

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330-EN479

   Plasmid

   #86234

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse rosa26 locus
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-His-TEV-NQO1

   Plasmid

   #119058

   Purpose

   For expression in E.coli and affinity purification of N-terminally His tagged NQO1

   Depositor

   Yosef Shaul

   Article

   Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

   Insert

   NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
   

   Use

   Tags

   6His, TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJMP2

   Plasmid

   #79874

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into amyE

   Depositor

   Carol Gross, Jason Peters, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-C1-BBS2

   Plasmid

   #218712

   Purpose

   Expresses N-terminally EGFP-tagged BBS2 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   BBS2 (BBS2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-EGFP-C1-HsEDC3

   Plasmid

   #25032

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   EDC3 (EDC3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CFAST-Halotag-6xHp

   Plasmid

   #214416

   Purpose

   Lipid droplet localization of CFAST fused to Halotag-6xHp

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   deletion
   

   Use

   Tags

   CFAST-Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABE hygro MEN1 WT

    Plasmid

    #11024

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

    Plasmid

    #193752

    Purpose

    human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-FAB(ER-mito)

    Plasmid

    #214423

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
    

    Use

    Lentiviral

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5 FLAG wildtype, catalytic active lipin 1

    Plasmid

    #32005

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

    Plasmid

    #176742

    Purpose

    Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Aqp4-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.pU1a-SpCas9

    Plasmid

    #121507

    Purpose

    Expresses codon-optimized SpCas9 in mammalian cells.

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    SpCas9
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA WDR24 pRK5

    Plasmid

    #46335

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR-cBEST-v2-PermE

    Plasmid

    #209446

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from ermE* promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEntry_HCoV-229E-Spike

    Plasmid

    #168971

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-Spike (S )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PAmCherry1-C1-LACT-C2

    Plasmid

    #202682

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    PAmCherry1: Bos taurus MFGE8 (274-431) (MFGE8 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TDP-43_CTD_Q331K

    Plasmid

    #98673

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331K

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Q331K

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge