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pLentiX1-[TRE3G-TS.FF4x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235316

Purpose

Lentiviral expression of mRuby2 with 5' microRNA target site only (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF6x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235323

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF6x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235315

Purpose

Lentiviral expression of mRuby2 with 5' microRNA target site only (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF6x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235321

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235313

Purpose

Lentiviral expression of mRuby2 with 3' microRNA target site only (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235319

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235314

Purpose

Lentiviral expression of mRuby2 with 3' microRNA target site only (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF4x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235322

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF4x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235324

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-AtmiR173aTS-B/c

Plasmid

#227964

Purpose

Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge