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CRISPRi sgNF1-1

Plasmid

#253096

Purpose

CRISPRi single-guide against NF1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

NF1 (NF1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgSOS1-1

Plasmid

#253092

Purpose

CRISPRi single-guide against SOS1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

SOS1 (SOS1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-ATG13-HF|DD

Plasmid

#249535

Purpose

Expressing human full length ATG13 with H214D/F215D mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H214D and F215D

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ATG101ΔCTH (WF|DD)

Plasmid

#250341

Purpose

Expressing human ATG101ΔCTH with W110D and F112D mutation

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG101 (ATG101 Human)

Tags

strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG101(WF|DD)

Plasmid

#250342

Purpose

Expressing human full length ATG101 with W110D and F112D mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG101 (ATG101 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-ATG13-1-230

Plasmid

#250343

Purpose

Expressing human ATG13 (aa. 1-230)

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG13 (ATG13 Human)

Tags

GFP

Expression

Mammalian

Mutation

only amino acids 1-230

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAJE90-Ma-tfmW-RSA2

Plasmid

#251520

Purpose

Machinery plasmid expressing Methanomethylophilus alvus aaRS/tRNA for TAG suppression and incorporation of trifluoromethyl-tryptophan (tfm-Trp) into sfGFP150TAG in E. coli

Depositor

Ryan Mehl

Article

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

Ma-tfmW-RSA2

Expression

Bacterial

Promoter

Gln S

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pYO1704

Plasmid

#252704

Purpose

Protein expression of GST-HsLC3B (1-120) in bacteria

Depositor

Roger Williams

Article

van Vliet et al J Cell Biol. 2026 Mar 2;225(3):e20250708

Insert

GST-HsLC3B (1-120) (MAP1LC3B Human)

Expression

Bacterial

Mutation

1-120

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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IR InterIR gRNA #5

Plasmid

#247547

Purpose

negative control for Crispri KD

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

negative control sgRNA targeting region between inverted repeats of Distal Junction

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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InterIR gRNA #2

Plasmid

#247546

Purpose

negative control for Crispri KD

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

negative control sgRNA targeting region between inverted repeats of Distal Junction

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA mouse Fis1

Plasmid

#252270

Purpose

Knockdown of mouse Fis1

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

shRNA mouse Fis1 (Fis1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA mouse Fascin1

Plasmid

#252271

Purpose

Knockdown of mouse Fascin1

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

shRNA mouse Fascin1 (Fscn1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA human Fis1

Plasmid

#252272

Purpose

Knockdown of human Fis1

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

shRNA human Fis1 (FIS1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA human Fascin1

Plasmid

#252273

Purpose

Knockdown of hunam Fascin1

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

shRNA human Fascin1 (FSCN1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pAH_DEST_nHalo-Sec61β-3XFLAG

Plasmid

#250996

Purpose

Expression of Sec61β with N-terminal Halo and C-terminal 3XFLAG tags

Depositor

Anthony Pedley

Article

Sha et al Proc Natl Acad Sci U S A. 2026 Feb 3;123

Insert

SEC61β (SEC61B Human)

Tags

3XFLAG and Halo Tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A

Plasmid

#220237

Purpose

Expresses dCAS9-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A (dCas9-3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-Gal4UAS-PGK-BFP

Plasmid

#247505

Purpose

GAL4/UAS expression of BFP

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

GAL4/UAS expression of BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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TPC2-G-GECO1.2 (F90L)

Plasmid

#240470

Purpose

Lysosomal channel with a low-affinity GECI fused to the C-terminus

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

G-GECO1.2_F90L

Expression

Mammalian

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TPC2-D276K-mCherry

Plasmid

#240469

Purpose

Red-tagged lysosomal channel (pore-dead)

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Mutated aspartate 276 to lysine.

Promoter

CMV

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pWidom601.CDKN2Bp.601

Plasmid

#247019

Purpose

Two Widom601 sequences, with a short linker with sequence from the CDKN2B locus between them. For large scale PCR amplification of DNA for nucleosome refolding.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Widom601 sequences and CDKN2B locus

Use

Synthetic Biology

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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