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pRPL28(Q38P) SpHIS5 (pNTI664)

Plasmid

#115434

Purpose

Expresses Q38P RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

Q38P; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRPL28(H39L) SpHIS5 (pNTI665)

Plasmid

#115435

Purpose

Expresses H39L RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

H39L; MfeI site in intron

Promoter

ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA

Plasmid

#87572

Purpose

PV4 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA

Plasmid

#89056

Purpose

PV3 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW759_pCAG-PV2-lox2272-GFP-BGHpA

Plasmid

#87575

Purpose

PV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA

Plasmid

#87580

Purpose

PV1 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

Neomycin Resistance Gene

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAD06 K. lactis telomerase RNA hairpin

Plasmid

#61895

Purpose

Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin (nt 229-273)

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M1A500(KaganF60)

Plasmid

#52003

Purpose

Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A-500

Expression

Mammalian

Mutation

changed Met 1 to Ala and deleted the C-terminal t…

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M1A,M142A-invitro(KaganE14)

Plasmid

#52018

Purpose

Used for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M1A,M142A(KaganE20)

Plasmid

#52011

Purpose

Expresses the human MAVS CDS containing the point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Retroviral

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only