We narrowed to 12,153 results for: 112

1. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a_6HT-TBP6.7(R47K)

   Plasmid

   #112723

   Purpose

   Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47K)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47K

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a_6HT-TBP6.7(Q48A)

   Plasmid

   #112724

   Purpose

   Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a_6HT-TBP6.7(T50A)

   Plasmid

   #112727

   Purpose

   Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(T50A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   T50A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TAP-Amotl2

    Plasmid

    #112834

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Insert

    Amotl2 (Amotl2 Mouse)
    

    Tags

    TAP

    Expression

    Mammalian

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)/Puro/FA/LL5BIP-GFP

    Plasmid

    #112831

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

    Insert

    LL5BIP (2410002F23Rik Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSYN-LL5BIP-GFP

    Plasmid

    #112847

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

    Insert

    LL5BIP (2410002F23Rik Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSYN-GFP-LL5BIP

    Plasmid

    #112846

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

    Insert

    LL5BIP (2410002F23Rik Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

    Plasmid

    #112987

    Purpose

    In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

    Insert

    mei-S332 (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Mutation

    S123-125-3A

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D

    Plasmid

    #112986

    Purpose

    P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331D) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

    Insert

    mei-S332 (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Mutation

    T331D

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 6xmyc_cort_UAS-P

    Plasmid

    #112991

    Purpose

    P element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Pesin et al PLoS Genet. 2007 Nov;3(11):e202. doi: 10

    Insert

    cort (Cdc20) (cort Fly)
    

    Use

    P insertion vector

    Tags

    6X myc epitope

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C-term png in pGEX4

    Plasmid

    #112975

    Purpose

    For protein expression and purification of the C-term of Drosophila png

    Depositor

    Terry Orr-Weaver

    Article

    Fenger et al Development. 2000 Nov;127(22):4763-74.

    Insert

    png (png Fly)
    

    Expression

    Bacterial

    Mutation

    wild-type C terminus

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C2 G10 png/GFP in casper4

    Plasmid

    #112970

    Purpose

    P element vector for transposon insertion of png-GFP into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Insert

    png-GFP (png Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX6P-1 BICC FL

    Plasmid

    #112998

    Purpose

    For protein expression and purification of full-length Drosophila BicC

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length BicC (BicC Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C2 png genomic in Casper

    Plasmid

    #112971

    Purpose

    P element vector for transposon insertion of png genomic DNA into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Fenger et al Development. 2000 Nov;127(22):4763-74.

    Insert

    png genomic DNA (png Fly)
    

    Use

    P insertion vector

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge