We narrowed to 863 results for: Px330

1. dCas12Max-ABE

   Plasmid

   #195335

   Purpose

   vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized dCas12Max-ABE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N243R, E336R, D1049A

   Promoter

   CBh, CMV, hU6

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. dCas12Max-CBE

   Plasmid

   #195337

   Purpose

   vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized dCas12Max-CBE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N243R, E336R, D1049A

   Promoter

   CBh, CMV, hU6

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196076

   Purpose

   (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196074

   Purpose

   (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196082

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   Aminoglycoside phosphotransferase
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196080

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   Blasticidin S deaminase
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cas9_P2A_T4pol

   Plasmid

   #190146

   Purpose

   Expresses SpCas9 and T4pol

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_T4pol
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SpCas9 dFE

   Plasmid

   #190137

   Purpose

   Expresses SpCas9-T4pol (dFE)

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9-T4pol (dFE)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cas9_P2A_POLB

   Plasmid

   #190145

   Purpose

   Expresses SpCas9 and POLB

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_POLB
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SpCas9 iFE

    Plasmid

    #190138

    Purpose

    Expresses SpCas9-POLB (iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9-POLB (HiFi iFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HiFi dFE

    Plasmid

    #190142

    Purpose

    Expresses HiFiCas9-T4pol (HiFi dFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    HiFi Cas9-T4pol (HiFi dFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    SpCas9 R691A mutant, Codon Optimized for mammalia…

    Promoter

    CBh

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HiFi iFE

    Plasmid

    #190141

    Purpose

    Expresses HiFiCas9-POLB (HiFi iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    HiFi Cas9-POLB
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    SpCas9 R691A mutant, Codon Optimized for mammalia…

    Promoter

    CBh

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTK473

    Plasmid

    #114715

    Purpose

    sgRNA for LGN's N-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    LGN sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTK372

    Plasmid

    #114687

    Purpose

    sgRNA targeting NuMA's C-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    NuMA sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTK308

    Plasmid

    #114689

    Purpose

    sgRNA targeting DHC's C-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    DHC-C sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTK525

    Plasmid

    #114691

    Purpose

    sgRNA for p150's C-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    p150 sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTK371

    Plasmid

    #114693

    Purpose

    sgRNA for DHC's N-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    DHC-N sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYMCR2_Gb_PMLTSS

    Plasmid

    #140285

    Purpose

    CRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-in

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX300A-G+Nanog

    Plasmid

    #140280

    Purpose

    CRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locus

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN455

    Plasmid

    #137872

    Purpose

    Expression of gRNA targeting POGZ for CRISPRa; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge