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Showing: 841 - 860 of 87212 results

  5. GS2.1/EC

    Plasmid
    #132568
    Purpose
    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).
    Depositor
    Mathias Pribil
    Article
    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111
    Insert
    egg cell promoter, Cas9-mApple, pds3 sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    A. thaliana egg cell promoter
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    132568-plasmid-map-sequence-id-262634

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  7. pCMV-RR-ABE8e-V106W

    Plasmid
    #193656
    Purpose
    Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    dLbCas12a-TadA*8e-V106W, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K595R, TadA*8e-V106W
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193656-plasmid-map-sequence-id-422133

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  11. pCMV-RR-A3A-N57G

    Plasmid
    #193654
    Purpose
    Expression of base editor RR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RR-dLbCas12a-hA3A-N57G, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K595R, hAPOBEC3A-N57G
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193654-plasmid-map-sequence-id-422067

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  12. pCMV-RR-A3A-Y130F

    Plasmid
    #193655
    Purpose
    Expression of base editor RR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RR-dLbCas12a-hA3A-Y130F, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K595R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193655-plasmid-map-sequence-id-422124

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  13. pCMV-RVR-ABE8e-V106W

    Plasmid
    #193659
    Purpose
    Expression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-TadA*8e-V106W, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, TadA*8e-V106W
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193659-plasmid-map-sequence-id-422134

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  15. pCMV-enCas12a-A3A-Y130F

    Plasmid
    #193652
    Purpose
    Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    dLb-enCas12a-hA3A-Y130F, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193652-plasmid-map-sequence-id-422135

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  16. pCMV-RVR-A3A-Y130F

    Plasmid
    #193658
    Purpose
    Expression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-hA3A-Y130F, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    193658-plasmid-map-sequence-id-426532

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  17. pCMV-enCas12a-A3A-N57G

    Plasmid
    #193651
    Purpose
    Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    dLb-enCas12a-hA3A-N57G, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193651-plasmid-map-sequence-id-422137

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  18. pCMV-RVR-A3A-N57G

    Plasmid
    #193657
    Purpose
    Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-hA3A-N57G, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193657-plasmid-map-sequence-id-422142

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  19. pVZ_PcoaT_SQE1_THAS1

    Plasmid
    #103859
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    thalianol synthase 1 (THAS1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103859-plasmid-map-sequence-id-217087

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Showing: 841 - 860 of 87212 results