We narrowed to 7,233 results for: mag

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pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBH-UAS-secA5-YFP

Plasmid

#32359

Purpose

Live imaging of apoptotic cells in zebrafish

Depositor

Randall Peterson

Article

van Ham et al FASEB J. 2010 Nov;24(11):4336-42. Epub 2

Insert

secreted human annexin 5 (ANXA5 Human)

Use

Zebrafish expression

Promoter

UAS

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-S in pAR9 (rep/cap)

Plasmid

#174539

Purpose

encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

Depositor

Casey Maguire

Article

Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

Insert

VP1 of AAV9 containing the AAV-S 7-mer peptide insert

Use

AAV

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-loxP-STOP-loxP-Venus

Plasmid

#170520

Purpose

Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Article

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-loxP-STOP-loxP-Venus

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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ArcLight-Q239

Plasmid

#36856

Depositor

Vincent Pieribone

Article

Jin et al Neuron. 2012 Sep 6;75(5):779-85.

Insert

fluorescent protein voltage sensor

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

simian CMV

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pDIV272

Plasmid

#226183

Purpose

Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD/His-B-iRFP

Plasmid

#31855

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Expression

Bacterial

Mutation

S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

Promoter

araBAD

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-Nedd4/2795

Plasmid

#11426

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

D661N compared to GenBank reference sequence U966…

Promoter

CMV

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-FLIM-AKAR

Plasmid

#63058

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV

Expression

Mammalian

Promoter

EF-1α

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP

Plasmid

#32481

Purpose

Cre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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CAG sHRPb-FKBP-post mGRASP

Plasmid

#73146

Purpose

Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-FKBP-post mGRASP

Tags

HA tag

Expression

Mammalian

Promoter

CAG

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DAKAP-μDpTT

Plasmid

#182324

Purpose

Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

DAKAP-μDronpa-TagRFP-T

Tags

N-terminal 30 amino acids from DAKAP1

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAGFP

Plasmid

#58255

Purpose

U6 promoter-driven single guide RNA complementary to eGFP

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAGFP cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern/pcDNA3

Plasmid

#67661

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Expression

Mammalian

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

Plasmid

#32479

Purpose

Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F-GlyR-IRES-GFP

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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Cyan Nano-lantern/pcDNA3

Plasmid

#67660

Purpose

cyan luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern

Expression

Mammalian

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pK_P-GAP-ACC1*

Plasmid

#126740

Purpose

yeast plasmid overexpressing ACC1 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acc1*

Expression

Yeast

Mutation

T3452G

Promoter

GAP

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(-)2G

Plasmid

#62799

Purpose

mtZFN2G architecture bearing (-) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(-)2G

Tags

Internal FLAG tag immediately after F1B mitochond…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(+)2G

Plasmid

#62798

Purpose

mtZFN2G architecture bearing (+) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(+)2G

Tags

Internal HA tag immediately after F1B mitochondri…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDronpa-N1-Tau-μDpTT

Plasmid

#182323

Purpose

Microtubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Tau-μDronpa-TagRFP-T

Tags

MAP tau

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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