Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,947 results for: 112

Showing: 8581 - 8600 of 12947 results

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pHRSV_CHARMS-DHF

Plasmid

#233922

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed Helical Filament)

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-DHF

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-1x

Plasmid

#233923

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-1x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-3x

Plasmid

#233925

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifs

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-3x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-5x

Plasmid

#233927

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifs

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-5x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS_bDD

Plasmid

#233931

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-dDD contains the bacterial death domain from Nostoc.

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-bDD

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-11xTA

Plasmid

#233932

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 11 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-11xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-15xTA

Plasmid

#233933

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-15xTAcontains 15 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-15xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-17xTA

Plasmid

#233934

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTAcontains 17 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-17xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-20xTA

Plasmid

#233935

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-10xTAcontains 20 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-20xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-TIR

Plasmid

#233919

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS): CHARMS-TIR

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-TIR

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3220

Plasmid

#234099

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfGFP

Tags

FLAG

Expression

Bacterial

Mutation

S30R/Y39N/N105T/Y145F/I171V/A206V

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3237

Plasmid

#234091

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfGFP

Tags

FLAG

Expression

Bacterial

Mutation

S30R/Y39N/N105T/Y145F/I171V/A206V

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N73_eMagBF_V5

Plasmid

#235642

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_Fbl_N73_eMagBF_V5

Plasmid

#235643

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, Fbl for targeting to nucleolus.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

Fbl-N73- and eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_L31_CRISPR_downstream_sgRNA

Plasmid

#235610

Purpose

Encodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus downstream sgRNA

Use

CRISPR

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_L31_CRISPR_upstream_sgRNA

Plasmid

#235609

Purpose

Encodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus upstream sgRNA

Use

CRISPR

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N73_eMagBF_V5_NES

Plasmid

#235614

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-73

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_BirA

Plasmid

#235634

Purpose

Encodes full-length BirA fused to a nuclear export sequence and V5. Used as a positive control in ALIBi experiments

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

BirA

Tags

NES-V5

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N65_eMagBF_V5_NES

Plasmid

#235612

Purpose

Encodes ALIBi N-terminal fragment, residues 1-65, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-65

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N77_eMagBF_V5_NES

Plasmid

#235616

Purpose

Encodes ALIBi N-terminal fragment, residues 1-77, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-77

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 8581 - 8600 of 12947 results

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