We narrowed to 35,536 results for: kana

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WESSV strain SAH177 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228659

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Wesselsbron virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

Wesselsbron virus portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SPOV strain SM-6 V-1 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228656

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Spondweni virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

Spondweni virus portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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DENV-4 strain H241 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228652

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 4 for biochemical assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 4 portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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DENV-2 strain Thailand/16681/84 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228650

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 2 for biochemical assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 2 portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain

Plasmid

#228643

Purpose

Bacterial expression of the SARS-CoV-2 nsp3 macrodomain for crystallization of SARS-CoV-2 nsp3 macrodomain in P 4 3

Depositor

ASAP Discovery, Frank von Delft

Insert

SARS-CoV-2 NSP3 macrodomain (ORF1ab SARS-CoV-2)

Tags

His-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag

Plasmid

#228635

Purpose

Bacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interaction

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus A71 3C protease isoform A

Tags

His-Avi

Expression

Bacterial

Available Since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag

Plasmid

#228634

Purpose

Bacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interaction

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus Coxsackievirus A71 2A protease

Tags

His-Avi

Expression

Bacterial

Available Since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET28_Strep-Psc-pScaC

Plasmid

#228834

Purpose

E. coli expression of the ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

Strep

Expression

Bacterial

Mutation

aa 33-223

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK103

Plasmid

#226803

Purpose

Integrates KanR into NS1, 500 bp homology, one homologous HIP1 site in left homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK104

Plasmid

#226804

Purpose

Integrates KanR into NS2, 500 bp homology, one homologous HIP1 site in right homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK105

Plasmid

#226805

Purpose

Integrates KanR into glpK, 570 bp homology, three homologous HIP1 sites in left homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_pScaC(50-159)

Plasmid

#228836

Purpose

E. coli expression of a truncated ScaC passenger domain (aa 50-159)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

Strep

Expression

Bacterial

Mutation

aa 50-159

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK74

Plasmid

#226657

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, KanR, contains HIP1 site

Depositor

David Nielsen

Article

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pVP096

Plasmid

#222043

Purpose

pGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

mRFP1e

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCF

Plasmid

#216326

Purpose

PUlaA promoter driving expression of sfgfp

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

Superfolder green fluorescent protein

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTW358c

Plasmid

#227934

Purpose

Express Aβ42 F19S/L34P-T7n

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Aβ42-T7n

Expression

Bacterial

Mutation

F19S and L34P mutations in Aβ42

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDL1530

Plasmid

#226636

Purpose

This plasmid is for the induced expression of nrsB (formerly known as ORF229) from ICP1_2001.

Depositor

Andrew Camilli

Article

Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

Insert

nrsB (ORF 229)

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAM299

Plasmid

#224258

Purpose

MCMUbHis

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MC-Ub-3C-His

Tags

His

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-mScarlet-I(+5)

Plasmid

#219418

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mScarlet-I(+5)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

D125K, D186K, E216K, E238K

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

Plasmid

#222861

Purpose

This plasmid codes for the OgeuIscB nickase

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB Nickase

Use

CRISPR

Tags

3X HA and Nucleoplasmin NLS and ITR2

Expression

Mammalian

Mutation

E193A for nickase mutation

Promoter

CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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